ALBERTO DIASPRO

Optical scanning system, comprising an optical system for guiding a first and a second light beam, and deflector devices for deflecting first and second light beams in a directionally variable manner. The deflector devices comprise at least one acousto-optic deflector, and the optical system is arranged in such a way that the first and second...

Lightening method of at least one biological sample (S), in which said at least one biological sample includes at least one or more fluorophores, at the focal point (F) of at least one objective lens (L) having a main optical axis (zz), the method comprising the following operational steps:—lightening (step 10) said at least one biological...

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Method of optical microscopy by scanning a sample containing an excitable species, the method comprising: directing a first and a second light beam onto respective, partially overlapped areas of the sample, wherein the first light beam is provided for exciting members of the excitable species, and the second light beam is provided for reducing...

Super-resolution microscopy is leading to important new advances in biomedicine and in those elds dealing with the study of biological systems. In many ways, it is the heir to two-photon microscopy, which itself represented a big revolution over a decade ago. We have put together this book to showcase these new advances and indicated how they...

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This work rationalizes the scalable synthesis of ultrasmall, ligand-free silicon nanomaterials via liquid-phase pulsed laser ablation process using picosecond pulses at ultraviolet wavelengths. Results showed that the irradiation time drives hydrodynamic NP size. Isolated, monodisperse Si-NPs are obtained at high yield (72%) using...

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Expansion microscopy (ExM) is a novel preparation method enhancing the optical resolution by expanding uniformly the relative distance between fluorescence molecules on a sample placed inside a polymerized gel matrix. However, a skilled operator is needed for fluorescent labeling protocols and a high light dose is required for measurement. In...

Dynamic biological systems present challenges to existing three-dimensional (3D) optical microscopes because of their continuous temporal and spatial changes. Most techniques are rigid in adapting the acquisition parameters over time, as in confocal microscopy, where a laser beam is sequentially scanned at a predefined spatial sampling rate and...