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AMBROGGIO , Ernesto

Last name: AMBROGGIO

First name: Ernesto

January 20, 2010 (v1)

Journal article

Metadata-only

ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions.

Ambroggio, Ernesto Sorre, Benoît Bassereau, Patricia

ArfGAP1, which promotes GTP hydrolysis on the small G protein Arf1 on Golgi membranes, interacts preferentially with positively curved membranes through its amphipathic lipid packing sensor (ALPS) motifs. This should influence the distribution of Arf1-GTP when flat and curved regions coexist on a continuous membrane, notably during COPI vesicle...

Uploaded on: December 3, 2022
November 4, 2008 (v1)

Journal article

Metadata-only

COPI coat assembly occurs on liquid-disordered domains and the associated membrane deformations are limited by membrane tension.

Manneville, Jean-Baptiste Casella, Jean-François Ambroggio, Ernesto

Cytoplasmic coat proteins are required for cargo selection and budding of tubulovesicular transport intermediates that shuttle between intracellular compartments. To better understand the physical parameters governing coat assembly and coat-induced membrane deformation, we have reconstituted the Arf1-dependent assembly of the COPI coat on giant...

Uploaded on: December 4, 2022