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ARROYOS , Aurelio

Last name: ARROYOS

First name: Aurelio

July 27, 2017 (v1)

Publication

Metadata-only

Direct Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis in Auramine-Rhodamine-Positive Sputum Specimens by Real-Time PCR

Ruiz Pérez, Maite Torres Sánchez, María José Llanos Rodríguez, Ana Cristina

Our objective was to evaluate the feasibility of a molecular assay based on a real-time PCR technique, carried out with a LightCycler instrument (Roche Biochemicals), to identify Mycobacterium tuberculosis bacilli and to detect rifampin and isoniazid resistance in DNA extracts from sputum samples. We studied three genes: rpoB, which is...

Uploaded on: December 4, 2022