Target of rapamycin complex 1 (TORC1), which controls growth in response to nutrients, promotes ageing in multiple organisms. The fission yeast Schizosaccharomyces pombe emerges as a valuable genetic model system to study TORC1 function and cellular ageing. Here we exploited the combinatorial action of rapamycin and caffeine, which inhibit...
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April 11, 2017 (v1)PublicationUploaded on: December 4, 2022
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May 18, 2017 (v1)Publication
In the fission yeast Schizosaccharomyces pombe, the stationary phase‐specific transcription factor Phx1 contributes to long‐term survival, stress tolerance, and meiosis. We identified Phx1‐dependent genes through transcriptome analysis, and further analyzed those related with carbohydrate and thiamine metabolism, whose expression decreased in...
Uploaded on: December 4, 2022 -
April 11, 2017 (v1)Publication
Background The fission yeast Schizosaccharomyces pombe is a popular genetic model organism with powerful experimental tools. The thiamine-regulatable nmt1 promoter and derivatives, which take >15 hours for full induction, are most commonly used for controlled expression of ectopic genes. Given the short cell cycle of fission yeast, however, a...
Uploaded on: December 5, 2022 -
April 11, 2017 (v1)Publication
Deletion of the sla1+ gene, which encodes a homologue of the human RNA-binding protein La in Schizosaccharomyces pombe, causes irregularities in tRNA processing, with altered distribution of pre-tRNA intermediates. We show, using mRNA profiling, that cells lacking sla1+ have increased mRNAs from amino acid metabolism (AAM) genes and,...
Uploaded on: March 27, 2023