BASSI , Anna Maria

On September 29-30, 2016, the 8th edition of the training course on "Alternative Approach to Animal Testing and Cell Cultures, According to European Laws", was held in Genoa, chaired by Prof. Anna Maria Bassi and organized and hosted by the team of Laboratory Analysis and Research in Physiopathology (LARF), Department of Experimental ...

No description

This study examines how treatment with a single dose of thioacetamide, a known experimental hepatotoxin, alters the content of dolichol, dolichol isoprene units and retinol in isolated rat parenchymal and non-parenchymal liver cells at different times and when the animals are supplemented with Vitamin A. Thioacetamide (300 mg/kg i.p.) was...

The aim of this paper was to ascertain whether chronic pretreatment with thioacetamide (TAA) might alter the uptake of a load of retinol and dolichol distribution in hepatocytes (HC), hepatic stellate cells (HSC) (Ito-1 and Ito-2 subfractions), Kupffer (KC) and sinusoidal endothelial cells (SEC). The reason why retinol and dolichol content was...

the hypothesis that dolichol and retinol metabolism might be interrelated and involved in liver fibrosis was investigated in this study. To this end, rats were subjected to chronic treatment with thioacetamide (TAA) (300 mg/L liquid diet) for 1 and 2 months and, after liver damage had occurred, supplemented with vitamin A before sacrifice....

Modulation of antioxidant status in a macrophage cell line during chronic exposure to glycated serum

No description

interview to Author for winning Lush Prize 2013 for the efforts to encourage training programmes aimed at the dissemination of the knowledge,information and skills needed to promote and support the increased use of available andaccepted non-animal test methods and testing strategies by regulators and end-users in industryand academia.

Urinary tract infections (UTIs) are frequently polymicrobial diseases mainly sustained by Escherichia coli in association with other opportunistic pathogens. Cystitis and pyelonephritis are usually accompanied by an inflammatory response, which includes neutrophil recruitment. Uropathogenic E. coli possess the ability to evade host defenses,...

No description

No description

Effects of Association of thioacetamide and ethanol treatment in isolated rat liver cells: evaluation of dolichol and retinol icontents

The aim of this study was to use chronic ethanol intoxication for 2 and 4 months as a means of studying the distribution of dolichol and retinol in isolated rat liver parenchymal cells, Kupffer cells, sinusoidal endothelial cells, and two subfractions of hepatic stellate cells: Ito 1 and Ito 2. Dolichol and retinol were studied in two batches...

No description

La complementarità del test in vitro rispetto alle valutazioni in vivo risiede nel fatto di poter arricchire il test in vivo di parametri quantitativi, sensibili, precoci e spesso unici perché non esplorabili direttamente sull"uomo. Il test in vitro può impiegare sistemi biologici semplificati (colture cellulari) o sofisticati (organi e tessuti...

Aim of this study was to verify the action, in rats in vivo, of the protein isoprenylation inhibitor perillyl alcohol (POH) and that of vitamin A, alone or in association, on m-RNA and protein expression of farnesyltransferases (FTases a and b subunits) and their protein substrates RhoA and RhoB, in isolated hepatocytes. Combined administration...

Hepatoma cell cultures as in vitro models for the hepatotoxicity of xenobiotics

No description