BABIANO GONZÁLEZ , Reyes

En el Capítulo I de esta tesis se muestran los resultados de la caracterización funcional de la proteína ribosómica L35, componente esencial de la subunidad grande del ribosoma. Nuestros resultados indican que la ausencia de L35 en las células tiene como consecuencia un déficit en la producción de subunidades 60S. Tal y como se demuestra por...

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The synthesis of cytoplasmic eukaryotic ribosomes is an extraordinarily energy-demanding cellular activity that occurs progressively from the nucleolus to the cytoplasm. In the nucleolus, precursor rRNAs associate with a myriad of trans-acting factors and some ribosomal proteins to form pre-ribosomal particles. These factors include snoRNPs,...

Ribosomal proteins play important roles in ribosome biogenesis and function. Here, we study the evolutionarily conserved L26 inSaccharomyces cerevisiae, which assembles into pre-60S ribosomal particles in the nucle(ol)us. Yeast L26 is one of the many ribosomal proteins encoded by two functional genes. We have disrupted both genes; surprisingly,...

Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A) + -RNA– binding protein Nab2, which regulates the mRNA poly(A) + -tail length and export. Here we show that Nab2 has additional roles during mRNA transcription, tRNA...

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During translation, nascent polypeptide chains travel from the peptidyl transferase center through the nascent polypeptide exit tunnel (NPET) to emerge from 60S subunits. The NPET includes portions of five of the six 25S/5.8S rRNA domains and ribosomal proteins uL4, uL22, and eL39. Internal loops of uL4 and uL22 form the constriction sites of...

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