BARBIER , Olivier

The aim of this study was to characterize the mechanism implicated in Zn(2+) transport in MDCK cells. Trace elements such as Zn(2+), Cd(2+) or Cu(2+) induced MDCK cell depolarization at the micromolar level as demonstrated by bis-oxonol fluorescence and whole-cell patch experiments. This depolarization was inhibited by La(3+) and Gd(3+) and was...

Heavy metals such as cadmium (Cd), mercury (Hg), lead (Pb), chromium (Cr) and platinum (Pt) are a major environmental and occupational hazard. Unfortunately, these non-essential elements are toxic at very low doses and non-biodegradable with a very long biological half-life. Thus, exposure to heavy metals is potentially harmful. Because of its...

BACKGROUND: The aim of this work was to characterize the relationship between zinc (Zn(2+)) and cadmium (Cd(2+)) and the toxic effects of Cd(2+) in immortalized renal proximal tubule cells RP1. METHODS: An RP1 cell line was developed from primary cultures of microdissected S1 and S2. Uptakes of (65)Zn and (109)Cd and competitive experiments...