BARELLI , Hélène

Background: Previous studies have shown that Alzheimer's disease (AD) is characterised by significant alterations of omega6 and omega3 polyunsaturated chains (PC) incorporated in phospholipids. Investigating the dynamics of PC changes during the natural history of AD, in the brain as well as in the blood, is key for highlighting the role of...

International audience

Nous ́etudions la validit ́e des mod'eles de r ́eaction-diffusion de type Fisher-KPP pour la simulation de migration de feuillets cellulaires. Pour cela, nous avons effectu ́e des observations exp ́erimentales sur les monocouches de cellules MDCK. Les videoscopies obtenues sont segment ́ees et binaris ́ees, permettant d'obtenir avec pr ́ecision...

We address in this paper the ability of the Fisher-KPP equations to render some of the dynamical features of epithelial cell-sheets during wound closure. Our approach is based on nonlinear parameter identification, in a two-dimensional setting, and using advanced 2D image processing of the video acquired sequences. As original contribution, we...

We address in this paper the ability of the Fisher-KPP equations to render some of the dynamical features of epithelial cell-sheets during wound closure. Our approach is based on nonlinear parameter identification, in a two-dimensional setting, and using advanced 2D image processing of the video acquired sequences. As original contribution, we...

International audience

International audience

Nous ́etudions la validit ́e des mod'eles de r ́eaction-diffusion de type Fisher-KPP pour la simulation de migration de feuillets cellulaires. Pour cela, nous avons effectu ́e des observations exp ́erimentales sur les monocouches de cellules MDCK. Les videoscopies obtenues sont segment ́ees et binaris ́ees, permettant d'obtenir avec pr ́ecision...

International audience

International audience

International audience

Proteins of the cytohesin/Arno/Grp1 family of Arf activators are positive regulators of the insulin-signaling pathway and control various remodeling events at the plasma membrane. Arno has a catalytic Sec7 domain, which promotes GDP to GTP exchange on Arf, followed by a pleckstrin homology (PH) domain. Previous studies have revealed two...

Recent reports have shown that amyloid beta deposits in the brains of Alzheimer's disease patients consist mainly of two distinct species of amyloid beta protein (Abeta) with different C-termini, Abeta1-42 (Abeta42) and Abeta1-40 (Abeta40). The nature of the Abeta species in Microcebus murinus brain was investigated immunocytochemically using...

Some mammalian cells show striking differences in the acyl chain composition of their membrane phospholipids. In most cases, the majority of phospholipids bear one saturated and one monounsaturated acyl chains at positions 1 and 2 or the glycerol, respectively. However, some cells and notably neurons contain large amounts of phospholipids with...

Some mammalian cells show striking differences in the acyl chain composition of their membrane phospholipids. In most cases, the majority of phospholipids bear one saturated and one monounsaturated acyl chains at positions 1 and 2 or the glycerol, respectively. However, some cells and notably neurons contain large amounts of phospholipids with...

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International audience

Small molecules that produce nonfunctional protein-protein complexes are an alternative to competitive inhibitors for the inhibition of protein functions. Here we target the activation of the small GTP-binding protein Arf1, a major regulator of membrane traffic, by the Sec7 catalytic domain of its guanine nucleotide exchange factor ARNO. The...

Small molecules that produce nonfunctional protein-protein complexes are an alternative to competitive inhibitors for the inhibition of protein functions. Here we target the activation of the small GTP-binding protein Arf1, a major regulator of membrane traffic, by the Sec7 catalytic domain of its guanine nucleotide exchange factor ARNO. The...

Phospholipids (PLs) with polyunsaturated acyl chains are extremely abundant in a few specialized cellular organelles such as synaptic vesicles and photoreceptor discs, but their effect on membrane properties is poorly understood. Here, we found that polyunsaturated PLs increased the ability of dynamin and endophilin to deform and vesiculate...

Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (PLs) in physiology. Here, we study the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) tunnels triggered by the RhoA GTPase inhibitory exotoxin C3 from Clostridium...

Metabolic studies and animal knockout models point to the critical role of polyunsaturated docosahexaenoic acid (22:6, DHA)-containing phospholipids (DHA-PLs) in physiology. Here, we investigated the impact of DHA-PLs on the dynamics of transendothelial cell macroapertures (TEMs) triggered by RhoA inhibition-associated cell spreading. Lipidomic...