BARNOIN , Guillaume

Recent discoveries of the involvement of nucleic acids in a wide range of biological processes have highlighted the importance of being able to detect and localise them precisely within cells. To achieve this, many strategies have been developed. The process of resonance energy transfer between two fluorescent molecules (FRET) has made a major...

The intensive research for hybridization probes based on organic molecules with fluorogenic properties is currently attracting particular attention due to their potential to efficiently recognize different DNA conformations and the local environment. However, most established organic chromophores do not meet the requirements of this task, as...

Forced intercalation (FIT) probes have proven to be a reliable, rapid, inexpensive, and accurate method for the detection and visualization of specific nucleic acid sequences. The accommodation of a rationally designed chromone-based fluorogen within a double-stranded DNA structure was investigated by UV-Vis spectrophotometry and steady-state...

In this paper, we report on the Photoinduced Electron Transfer (PET) reaction between a donor (adenine analog) and an acceptor (3-methoxychromone derivative) in the context of designing efficient fluorescent probes as DNA sensors. Firstly, Gibbs energy was investigated in disconnected donor-acceptor systems by Rehm-Weller equation. The...

Abstract The sensitivity of FRET-based sensing is usually limited by the spectral overlaps of the FRET donor and acceptor, which generate a poor signal-to-noise ratio. To overcome this limitation, a quenched donor presenting a large Stokes shift can be combined with a bright acceptor to perform Dark Resonance Energy Transfer (DRET). The...

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