BIANCHINI , Paolo

Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity. However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in...

Optical microscopy demonstrated an incredible power and ability in producing large data set originated from biological samples by light interrogation and tunable in terms of spatial at temporal resolution down to the nano- and pico- scale, respectively. Such a data set is the core for developing an artificial microscope aiming to transform a...

The desire for new methods that can explore the function of biological systems without the need for sample modification, or the addition of fluorescent tags, is steadily emerging. The development of so called 'label free' microscopies is important for the exploration of the natural state of biological systems, without alteration and without...

Transient absorption, or pump-probe microscopy is an absorption-based technique that can explore samples ultrafast dynamic properties and provide fluorescence-free contrast mechanisms. When applied to graphene and its derivatives, this technique exploits the graphene transient response caused by the ultrafast interband transition as the imaging...

Label free microscopic approaches have become a necessity to explore, image, and understand, complex biological systems with minimal sample modifications [1]. These innovative microscopes exploit the native photophysical processes present when light interacts with matter, presenting a plethora of options not only for introducing image contrast,...

In the last few decades fluorescence microscopy has been the most widely used microscopy technique and much effort has been put into the development of advanced super-resolution fluorescence microscopy techniques to circumvent the diffraction limit. Despite their well-established benefits, these techniques have to rely on the photo-physical...

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Three-dimensional imaging of cells or tissues is a crucial method in cellular and molecular biology due to its capability to identify and localise diverse biomolecules at the nanoscale. Recently, an approach has become very popular which can physically and isotropically expand a biological specimen through a chemical process by embedding the...