BIANCHINI P.

Two-photon excitation stimulated emission depletion nanoscopy (2PE-STED) is a fluorescence imaging technique ideal for significantly improving the spatial resolution when observing scattering tissue in fixed, in vitro, and in vivo specimens. Both 2PE and STED are beam scanning techniques. The image acquisition is commonly realized by raster...

No description

After publication of the article we noticed that equations number 24 and 25 missed a convolution sign.

Expansion microscopy (ExM) is a magnification method that allows achieving super-resolved images using a conventional light microscope. In ExM, biomolecules, fluorescent proteins, and dyes are functionalized with specific handles to link a dense polyelectrolyte hydrogel, which can achieve an isotropic expansion of 4.5-fold in water. The use of...

In this work, the preparation of a novel enzyme carrier based on a polymer multicomponent system was assessed. Indeed, the design of the above system considered several issues that are the need of applying a biodegradable polymer carrier, characterized by a nanometric dimension, thus suitable to diffuse into the dense mucus structure, with...

Light Sheet Microscopy (LSM), being capable of optical sectioning at diffraction-limited resolution and at low light doses [1], has become the tool of choice for volumetric imaging of large samples. Unfortunately, traditional LSM architectures are limited in speed because a z-stack is collected by either sample translation or synchronized...

Light-sheet microscopes have become the tool of choice for volumetric imaging of large samples. Based on a wide-field acquisition scheme, they are capable of optical sectioning at diffraction-limited resolution and minimal overall photodamage. Unfortunately, traditional architectures are limited in speed because 3D images are collected by...

The presence of background is a well-known problem in Single Molecule Localization Microscopy (SMLM). Indeed, the molecules localization precision depends quadratically on the background intensity. Here we try to isolate the single molecules removing the background through a scattering network representation and a Convolution Neural Network (CNN).

It is known that the presence of fluorophores can influence the dynamics of molecular processes. Despite this, an affordable technique to control the fluorophore distribution within the sample, as well as the rise of unpredictable anomalous processes induced by the fluorophore itself, is missing. We coupled a stimulated emission depletion...

No description