BISPO , Antonio

International audience

International audience

National audience

During the last two decades, novel molecular and robust methods were developed that were well-suited to characterize soil microbial communities, as they provided access to previously hidden genetic resources. These methods were based essentially on soil DNA characterization and most efforts were devoted to optimize the ...

During the last two decades, novel molecular and robust methods were developed that were well-suited to characterize soil microbial communities, as they provided access to previously hidden genetic resources. These methods were based essentially on soil DNA characterization and most efforts were devoted to optimize the ...

Soil is one of the most important reservoirs of microbiological diversity on our planet and, above all, one of the last bastions of such biodiversity. Since two decades, numerous molecular tools have been developed to assess accurately this huge diversity directly from soil DNA. In this context, 16S rRNA gene is widely used to study microbial...

Three soil DNA extraction procedures (homemade protocols and commercial kit) varying in their practicability were applied to contrasting soils to evaluate their efficiency in recovering: (i) soil DNA and (ii) bacterial diversity estimated by 16S rDNA pyrosequencing. Significant differences in DNA yield were systematically observed between...