BUTTAFAVA , M.

Method for operating a point laser- scanning microscope, comprising scanning a sample with a focused illumination laser beam (EB); recording, by means of an array of detecting elements (23), a plurality of images of the sample over a scan by the laser beam, wherein the detecting elements (23) are configurable to an intensity mode, in which the...

Stimulated emission depletion (STED) microscopy provides fluorescence imaging with sub-diffraction resolution. Experimentally demonstrated at the end of the 90s, STED microscopy has gained substantial momentum and impact only in the last few years. Indeed, advances in many fields improved its compatibility with everyday biological research....