CÁNOVAS LÓPEZ , David

Polarized growth is a defining property of filamentous fungi, which plays an important role in different aspects of their biology, including virulence. However, little information is available about the determinants of cell surface organization and their role in polarized growth. The fungal protein MesA was identified in a genetic screen in...

Durante la última década, nuestro Equipo de Investigación ha realizado numerosos estudios encaminados a un mejor conocimiento de la biología de las bacterias halófilas moderadas, procariotas que conjuntamente con las arqueas aerobias halófilas extremas, son los microorganismos más representativos de los ambientes hipersalinos. En concreto, las...

Cytokinesis is essential for proliferative growth but also plays equally important roles during morphogenesis and development. The human pathogen Penicillium marneffei is capable of dimorphic switching in response to temperature, growing in a multicellular filamentous hyphal form at 25°C and in a unicellular yeast form at 37°C. P. marneffei...

Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium, thereby...

Moderate osmolality can stimulate bacterial growth at temperatures near the upper limit for growth. We investigated the mechanism by which high osmolality enhances the thermotolerance of Salmonella enterica serovar Typhimurium, by isolating bacteriophage MudI1734-induced insertion mutations that blocked the growth-stimulatory effect of 0.2 M...

Penicillium marneffei is an opportunistic human pathogen endemic to Southeast Asia. At 25° P. marneffei grows in a filamentous hyphal form and can undergo asexual development (conidiation) to produce spores (conidia), the infectious agent. At 37° P. marneffei grows in the pathogenic yeast cell form that replicates by fission. Switching between...

Nitric oxide (NO) is a signalling molecule in eukaryotic and prokaryotic organisms. NO levels transiently boost upon induction of conidiation in Aspergillus nidulans. Only one pathway for NO synthesis involving nitrate reductase has been reported in filamentous fungi so far, but this does not satisfy all the NO produced in fungal cells. Here we...

Nitric oxide (NO) is a remarkable gaseous molecule with multiple and important roles in different organisms, including fungi. However, the study of the biology of NO in fungi has been hindered by the lack of a complete knowledge on the different metabolic routes that allow a proper NO balance, and the regulation of these routes. Fungi have...

Cepa Microbiana Terribacillus SP. AE2B 122 con capacidad para llevar a cabo reacciones de transesterificación y usos de la misma.La presente invención se refiere a una cepa bacteriana del género Terribacillus, denominada Terribacillus sp. AE2B 122 con cap

Background The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to...

The osmoregulatory pathways of the moderately halophilic bacterium Halomonas elongata DSM 3043 have been investigated. This strain grew optimally at 1.5 to 2 M NaCl in M63 glucose-defined medium. It required at least 0.5 M NaCl for growth, which is a higher concentration than that exhibited by the H. elongata type strain ATCC 33173. Externally...

The role of choline in osmoprotection in the moderate halophile Halomonas elongata has been examined. Transport and conversion of choline to betaine began immediately after addition of choline to the growth medium. Intracellular accumulation of betaine synthesized from choline was salt dependent up to 2.5 M NaCl. Oxidation of choline was...

The moderate halophile Halomonas elongata Deustche Sommlung fur Mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 M NaCl). Two Tn1732-induced mutants, CHR62 and CHR63, that were severely affected in their salt tolerance were isolated. Mutant CHR62 could not grow above 0.75 M...

Strain CHR63 is a salt-sensitive mutant of the moderately halophilic wild-type strain Halomonas elongata DSM 3043 that is affected in the ectoine synthase gene (ectC). This strain accumulates large amounts of N')'-acetyldiaminobutyrate (NADA), the precursor of ectoine (D. Ca´novas, C. Vargas, F. Iglesias-Guerra, L. N. Csonka, D. Rhodes, A....

Derivados acilados de carbohidratos, métodos de obtención y usos de los mismos. La presente invención describe derivados acilados de carbohidratos, preferentemente a los compuestos de fórmula 2,3,4,6,6'-penta-O-acetil-α,α-D-trehalosa o 2,3,4,6-tetra-O-acetil-α,α-D-trehalosa, así como el uso de los mismos como intermedios de reacción para...

Cepas bacterianas y sus usos en reacciones de acilación y/o desacilación. La presente invención describe cepas microbianas con capacidad de llevar a cabo reacciones de alcoholisis y esterificación regioselectivas y el uso de las mismas en la producción de carbohidratos y polifenoles lipófilos de interés en la industria farmacéutica y...

Cepas bacterianas y sus usos en reacciones de acilación y/o desacilación. La presente invención describe cepas microbianas con capacidad de llevar a cabo reacciones de alcoholisis y esterificación regioselectivas y el uso de las mismas en la producción de carbohidratos y polifenoles lipófilos de interés en la industria farmacéutica y...

Derivados acilados de polifenoles y/o carbohidratos, métodos de obtención y usos de los mismos. La presente invención describe derivados acilados de polifenoles y/o carbohidratos, métodos de obtención y usos de los mismos. Particularmente, la presente invención se refiere a los compuestos de fórmula general (I) que son de interés en la...

Cepas bacterianas y sus usos en reacciones de acilación y/o desacilación. La presente invención describe cepas microbianas con capacidad de llevar a cabo reacciones de alcoholisis y esterificación regioselectivas y el uso de las mismas en la producción de carbohidratos y polifenolas lipófilos de interés en la industria farmacéutica y...

Nitric oxide (NO) is a signalling molecule involved in many biological processes in bacteria, plants and mammals. However, little is known about the role and biosynthesis of NO in fungi. Here we show that NO production is increased at the early stages of the transition from vegetative growth to development in Aspergillus nidulans. Full NO...

Fossil fuels are consumed so rapidly that it is expected that the planet resources will be soon exhausted. Therefore, it is imperative to develop alternative and inexpensive new technologies to produce sustainable fuels, for example biodiesel. In addition to hydrolytic and esterification reactions, lipases are capable of performing...

Background: Fungi use light as an environmental signal to regulate developmental transitions that are key aspects of their biological cycles and that are also relevant for their dispersal and infectivity as plant or animal pathogens. In addition, light regulates the accumulation of photoprotective pigments, like carotenoids, and other...