Vertebrate retinal rod outer segments (OS) consist of a stack of disks surrounded by the plasma membrane, where phototransduction takes place. Energetic metabolism in rod OS remains obscure. Literature described a so-called Mg(2+)-dependent ATPase activity, while our previous results demonstrated the presence of oxidative phosphorylation...
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2013 (v1)PublicationUploaded on: April 14, 2023
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2013 (v1)Publication
Vertebrate retinal rod outer segments (OS) consist of a stack of disks surrounded by the plasma membrane, where phototransduction takes place. Energetic metabolism in rod OS remains obscure. Literature described a so-called Mg(2+)-dependent ATPase activity, while our previous results demonstrated the presence of oxidative phosphorylation...
Uploaded on: April 14, 2023 -
2008 (v1)Publication
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Uploaded on: March 31, 2023 -
2010 (v1)Publication
Snake venoms are complex toxin mixtures. Viperidae and Crotalidae venoms, which are hemotoxic, are responsible for most of the envenomations around the world. Administration of antivenins aimed at the neutralization of toxins in humans is prone to potential risks. Neutralization of snake venom toxins has been achieved through different...
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2013 (v1)Publication
The basic processes harvesting chemical energy for life are driven by proton (H(+)) movements. These are accomplished by the mitochondrial redox complex V, integral membrane supramolecular aggregates, whose structure has recently been described by advanced studies. These did not identify classical aqueous pores. It was proposed that H(+)...
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2012 (v1)Publication
White matter comprises over half of the brain, and its role in axonal survival is being reconsidered, consistently with the observation that axonal degeneration follows demyelization. The recent evidence of an extra-mitochondrial aerobic ATP production in isolated myelin vesicles, thanks to the expression therein of the mitochondrial Oxydative...
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2010 (v1)Publication
Glucose6phosphate dehydrogenase (G6PD) is a ubiquitous enzyme catalyzing the oxidation of Dglucose 6phosphate to Dglucono⎯lactone 6phosphate, in the first step of the pentose phosphate path way. Based on the currently available structural information on Leuconostoc mesenteroides G6PD, it is believed that the enzyme only works as a homodimer....
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2012 (v1)Publication
The pathogenesis of multiple sclerosis (MS), a disease characterized by demyelination and subsequent axonal degeneration, is as yet unknown. Also, the nature of the disease is as yet not established, since doubts have been cast on its autoimmune origin. Genetic and environmental factors have been implied in MS, leading to the idea of an overall...
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2011 (v1)Publication
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2004 (v1)Publication
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2010 (v1)Publication
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2009 (v1)Publication
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2005 (v1)Publication
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2012 (v1)Publication
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2007 (v1)Publication
Adenylate kinases (AKs) are ubiquitous monomeric phosphotransferases catalyzing the reversible reaction, AMP 1 MgATP = ADP 1 MgADP, which plays a pivotal role in the energetic metabolism. In vertebrates, six AK isoforms are known. In this work, we report the detection of many AK isoforms directly on gel or NC after separation by denaturing...
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2004 (v1)Publication
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2013 (v1)Publication
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2007 (v1)Publication
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2013 (v1)Publication
Myelin sheath is a lipid-rich membrane, consisting of 70\% lipid and 30\% proteins, that is involved in physiological and pathological processes. For this reason its protein composition has been often investigated, principally by two-dimensional electrophoresis; however, the consistent lipid content makes it difficult to obtain good proteins...
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2012 (v1)Publication
Vertebrate retinal rods are photoreceptors for dim-light vision. They display extreme sensitivity to light thanks to a specialized subcellular organelle, the rod outer segment. This is filled with a stack of membranous disks, expressing the proteins involved in visual transduction, a very energy demanding process. Our previous proteomic and...
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2013 (v1)Publication
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2014 (v1)Publication
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2010 (v1)Publication
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2010 (v1)Publication
We describe a procedure for the labeling of membranous vesicular purified subcellular fractions, to image them, typically by confocal laser scanning microscopy. Being intracellular organelles, these fractions, once purified cannot be attached to glass slides as for cells. Fractions are labeled ''in batch'' without prior embedding or freezing....
Uploaded on: April 14, 2023