CELLA , Francesca

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Diffraction imposes for each optical system a resolution limit which could be described by using the vectorial theory of Richards and Wolf. This theory defines the intensity distribution of a point like source imaged by a lens assuming ideal imaging conditions. Unfortunately, these conditions can not be completely achieved in practical...

In this article, we describe and show the application of some of the most advanced fluorescence superresolution techniques, STED AFM and STORM AFM microscopy towards imaging of cytoskeletal structures, such as microtubule filaments. Mechanical and structural properties can play a relevant role in the investigation of cytoskeletal structures of...

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In this work we report the advantages provided by two photon excitation (2PE) implemented in a selective plane illumination microscopy (SPIM) when imaging thick scattering samples. In particular, a detailed analysis of the effects induced on the real light sheet excitation intensity distribution is performed. The comparison between...

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The quantitative analysis of fluorescence perturbation experiments such as fluorescence recovery after photobleaching (FRAP) requires suitable analytical models to be developed. When diffusion in 31) environments is considered, the description of the initial condition produced by the perturbation (i.e., the photobleaching of a selected region...

Two-photon and confocal laser scanning microscopes represent fundamental optical techniques for biological investigations, thanks to their optical sectioning capability. However, in several experimental situations, low-intensity signals are detected, and the imaging quality can be remarkably limited by noise contribution. Such a detriment...

Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers...

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Light-sheet microscopy, such as ultramicroscopy, single plane illumination microscopy (SPIM) [1] and digital scanned laser microscopy (DSLM) [2], represents a useful tool for biological investigations of thick samples. Such techniques have been found particularly useful in developmental biology applications since they provide the capability to...