COLISTRA , Nicolo

Microelectrode arrays (MEAs) represent a powerful and popular tool to study in vitro neuronal networks and acute brain slices. The research standard for MEAs is planar or 2D-MEAs, which have been in existence for over 30 years and used for extracellular recording and stimulation from cultured neuronal cells and tissue slices. However, planar...

In this work, we present a simple technique for building interconnected 3-Dimensional (3D) neuronal networks coupled to Micro-Electrode Array (MEA) substrates. Particularly, we designed a confinement structure made of PDMS (poly-dimethyl-siloxane) for realizing two structurally and functionally interconnected 3D neuronal networks in vitro. The...