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2020 (v1)PublicationUploaded on: April 14, 2023
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2021 (v1)Publication
180a Wednesday, February 24, 2021 ptychographic module was developed and mounted on a commercial SIM, to simultaneously collect ptychography and SIM data. We show how changes in the morphology of a HGPS nucleus affect the phase compared to normal cells, and how the SIM-ptychography approach can increase the resolution compared with conventional...
Uploaded on: April 14, 2023 -
2019 (v1)Publication
Structured Illumination Microscopy (SIM) is an important tool among the Super Resolution Microscopy techniques [1]. SIM stands out for its capability to perform optical sectioning, multicolor channel acquisition and live cell imaging, thanks to its low phototoxicity. In SIM, the specimen is illuminated with a high-frequency grating pattern,...
Uploaded on: April 14, 2023 -
2021 (v1)Publication
The DEK oncoprotein is a ubiquitous nuclear factor that has been consistently associated with tumor progression: its expression level differs between normal and cancer cells, raising the possibility of using DEK as a tumor marker. Moreover, DEK is a non-histone architectural chromatin factor that may influence nucleosomal accessibility and...
Uploaded on: April 14, 2023 -
2023 (v1)Publication
Epigenetic dysregulation of chromatin is one of the hallmarks of cancer development and progression, and it is continuously investigated as a potential general bio-marker of this complex disease. One of the nuclear factors involved in gene regulation is the unique DEK protein-a histone chaperon modulating chromatin topology. DEK expression...
Uploaded on: February 4, 2024 -
2019 (v1)Publication
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Uploaded on: April 14, 2023 -
2020 (v1)PublicationAn Image-Based Approach to the Evaluation of Oncogene Activation Effects on Cell's Genomic Stability
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Uploaded on: April 14, 2023 -
2021 (v1)Publication
Stimulated emission depletion (STED) microscopy and Separation of Photons by Lifetime Tuning (SPLIT) provide optical super-resolution imaging, at the nanometer scale, on intact single cell nuclei. The resolution that can be achieved is limited by factors such as photobleaching and/or reduction of signal-to-noise, which depend on the conditions...
Uploaded on: April 14, 2023 -
2021 (v1)Publication
Oncogenes activation may lead to alterations in chromatin organization at the nanoscale by interfering with fundamental processes such as DNA transcription, DNA replication, and DNA Damage Response [1]. The in vitro observation of these molecular processes is crucial to understand how oncogenes can alter chromatin organization leading to tumor...
Uploaded on: April 14, 2023 -
2019 (v1)Publication
The architectural organization of chromatin can play an important role in gene expression by regulating the diffusion of molecules via binding interactions and molecular crowding [1]. In this respect, understanding how variations of chromatin architecture affect nuclear dynamics is of fundamental importance. Fluorescence Correlation...
Uploaded on: April 14, 2023 -
2020 (v1)Publication
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Uploaded on: April 14, 2023 -
2022 (v1)Publication
The molecular mechanisms that underlie oncogene-induced genomic damage are still poorly understood. To un-derstand how oncogenes affect chromatin architecture, it is important to visualize fundamental processes such as DNA replica-tion and transcription in intact nuclei and quantify the alterations of their spatiotemporal organization induced...
Uploaded on: February 4, 2024 -
2018 (v1)Publication
The possibility of integrating different light-matter interactions to form images in optical microscopy is the starting point for the design and realization of LIQUITOPY®, a ''liquid tunable microscope''. This could represent a new paradigm in data collection and image formation with a potential high impact in biophysics. Liquid takes...
Uploaded on: April 14, 2023 -
2019 (v1)Publication
Defects in the global organization of chromatin are relevant in many pathological processes, including cancer. The activation of oncogenes has been recognized as a triggering event of alterations in the dynamics of replication, transcription and DNA damage response (DDR), that may lead to increased DNA damage and the onset of genomic...
Uploaded on: April 14, 2023