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CASTRO QUEZADA , Patricio

Last name: CASTRO QUEZADA

First name: Patricio

2013 (v1)

Journal article

Metadata-only

Identification of reference genes for normalizing RNA expression in potato roots infected with cyst nematodes

Castro Quezada, Patricio Aarrouf, Jawad Claverie, Michel

Potato cyst nematodes induce changes in plant host gene expression following root invasion. For an accurate comparison of gene expression by reverse transcription quantitative real-time PCR (RT-qPCR), internal reference genes are necessary for transcript normalization. Very few experimental data on suitable reference genes are available for...

Uploaded on: December 4, 2022
June 5, 2012 (v1)

Publication

Metadata-only

Identification and validation of reference genes for normalization of transcripts levels in roots of potatoes infected by nematodes

Castro Quezada, Patricio Aarrouf, Jawad Claverie, Michel

Potato cyst nematodes induce changes in gene expression after infecting the roots. For studying gene expression, reverse transcription quantitative real-time PCR (RT-qPCR) is now a standard method. However, for accurate quantication of transcripts, a set of internal control genes, stable under different experimental conditions, is necessary for...

Uploaded on: December 4, 2022