CERUTTI , Elena

Confocal fluorescence microscopy is a well-established imaging technique capable of generating thin optical sections of biological specimens. Optical sectioning in confocal microscopy is mainly determined by the size of the pinhole, a small aperture placed in front of a point detector. In principle, imaging with a closed pinhole provides the...

Oncogenes activation may lead to alterations in chromatin organization at the nanoscale by interfering with fundamental processes such as DNA transcription, DNA replication, and DNA Damage Response [1]. The in vitro observation of these molecular processes is crucial to understand how oncogenes can alter chromatin organization leading to tumor...

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Stimulated emission depletion (STED) microscopy and Separation of Photons by Lifetime Tuning (SPLIT) provide optical super-resolution imaging, at the nanometer scale, on intact single cell nuclei. The resolution that can be achieved is limited by factors such as photobleaching and/or reduction of signal-to-noise, which depend on the conditions...

The molecular mechanisms that underlie oncogene-induced genomic damage are still poorly understood. To un-derstand how oncogenes affect chromatin architecture, it is important to visualize fundamental processes such as DNA replica-tion and transcription in intact nuclei and quantify the alterations of their spatiotemporal organization induced...

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In point-scanning microscopy, optical sectioning is achieved using a small aperture placed in front of the detector, i.e. the detection pinhole, which rejects the out-of-focus background. The maximum level of optical sectioning is theoretically obtained for the minimum size of the pinhole aperture, but this is normally prevented by the dramatic...