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Last name: CHATELAIN
First name: Franck
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Mechanosensitive K(+) channels TREK1 and TREK2 form a subclass of two P-domain K(+) channels. They are potently activated by polyunsaturated fatty acids and are involved in neuroprotection, anesthesia, and pain perception. Here, we show that acidification of the extracellular medium strongly inhibits TREK1 with an apparent pK near to 7.4...
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Ligand-gated ion channels (LGICs) are natural biosensors generating electrical signals in response to the binding of specific ligands. Creating de novo LGICs for biosensing applications is technically challenging. We have previously designed modified LGICs by linking G protein-coupled receptors (GPCRs) to the Kir6.2 channel. In this article, we...
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Among K2P channels, a few of them turned out tobe difficult to express in heterologous systems and werecoined "silent subunits". Recent studies have shed light onthe mechanisms behind this apparent lack of channel activityat the plasma membrane. For TWIK1 and THIK2 channels,silence is related to a combination of intracellular retentionand low...
Despite a high level of sequence homology, tandem poredomain halothane-inhibited K channel 1 (THIK1) producesbackground K currents, whereas THIK2 is silent. This lack ofactivity is due to a unique combination of intracellular retentionand weak basal activity in the plasma membrane. Here, wedesigned THIK subunits containing dominant negative...
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Inhibitory potassium channels of the TREK1/TRAAK family are integrators of multiple stimuli, including temperature, membrane stretch, polyunsaturated fatty acids and pH. How these signals affect the gating of these channels is the subject of intense research. We have previously identified a cytoplasmic domain, pCt, which plays a major role in...
Inhibitory potassium channels of the TREK1/TRAAK family are integrators of multiple stimuli, including temperature, membrane stretch, polyunsaturated fatty acids and pH. How these signals affect the gating of these channels is the subject of intense research. We have previously identified a cytoplasmic domain, pCt, which plays a major role in...
The tandem pore domain halothane-inhibited K channel 1(THIK1) produces background K currents. Despite 62% aminoacid identity with THIK1, THIK2 is not active upon heterologous expression. Here, we show that this apparent lack of activity is due to a unique combination of retention in the endoplasmic reticulum and low intrinsic channel activity...