COZZOLINO , Marco

Expansion microscopy (ExM) is a super-resolution imaging method that does not require any special optical microscope(1). The key point resides on the possibility of uniformly chemically expanding a sample, thus increasing the relative distances among objects of interest as fluorescent molecules labeling specific components. ExM is highly...

Expansion microscopy is a super-resolution method that allows expanding uniformly biological samples, by increasing the relative distances among fluorescent molecules labeling specific components. One of the main concerns in this approach regards the isotropic behavior at the nanoscale. The present study aims to determine the robustness of such...

RuBi GABA is a photoactivatable molecule whose activity can be controlled by a pulse of light. These probe compounds are prepared via covalent appendage of a light-sensitive protecting group "the cage" (RuBi) to a signaling molecule (GABA). With the cage bound, the signaling molecule is unable to activate its receptor [Diana D., J Am Chem Soc.,...

Photodynamic therapy (PDT) is a tumor treatment that uses a combination of otherwise non-toxic chemicals, termed photosensitizers (PSs) and visible light to produce reactive oxygen species capable of destroying tumor cells [Dougherty TJ (1993)][Agostinis P. et al.(2011)].PS molecules are often insoluble in water and form aggregates, which...

: The photodynamic treatment for antimicrobial applications or anticancer therapy relies on reactive oxygen species generated by photosensitizing molecules after absorption of visible or near-infrared light. If the photosensitizing molecule is in close vicinity of the microorganism or the malignant cell, a photocytotoxic action is exerted....