Las interacciones trasitorias entre biomoléculas se han convertido en el centro de la investigación de muchas áreas de la Bioquímica Moderna. La caracterización estructural continuada, utilizando cualquiera de las técnicas estructurales de alta resolución, es vital para comprender la función de este tipo de estos complejos. El trabajo de esta...
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November 27, 2014 (v1)PublicationUploaded on: March 25, 2023
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November 22, 2017 (v1)Publication
Identifying the factors that govern the thermal resistance of cupredoxins is essential for understanding their folding and stability, and for improving our ability to design highly stable enzymes with potential biotechnological applications. Here, we show that the thermal unfolding of plastocyanins from two cyanobacteria—the mesophilic...
Uploaded on: December 4, 2022 -
March 22, 2019 (v1)Publication
T-cell intracellular antigen-1 (TIA-1) plays a pleiotropic role in cell homeostasis through the regulation of alternative pre-mRNA splicing and mRNA translation by recognising uridine-rich sequences of RNAs. TIA-1 contains three RNA recognition motifs (RRMs) and a glutamine-rich domain. Here, we characterise its C-terminal RRM2 and RRM3...
Uploaded on: March 27, 2023 -
February 12, 2019 (v1)Publication
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Uploaded on: March 27, 2023 -
March 30, 2022 (v1)Publication
Cyclin M (CNNM1-4) proteins maintain cellular and body magnesium (Mg2+) homeostasis. Using various biochemical approaches, we have identified members of the CNNM family as direct interacting partners of ADP-ribosylation factor-like GTPase 15 (ARL15), a small GTP-binding protein. ARL15 interacts with CNNMs at their carboxyl-terminal conserved...
Uploaded on: December 4, 2022 -
January 18, 2018 (v1)Publication
Human antigen R (HuR) is a ubiquitous 32kDa protein comprising three RNA Recognition Motifs (RRMs), whose main function is to bind Adenylate and uridylate Rich Elements (AREs) in 3′ UnTranslated Regions (UTRs) of mRNAs. In addition to binding RNA molecules, the third domain (RRM3) is involved in HuR oligomerization and apoptotic signaling. The...
Uploaded on: December 4, 2022 -
August 21, 2017 (v1)Publication
The transient complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc sp. PCC 7119 has been analysed by X-ray Absorption Spectroscopy in solution, using both proteins in their oxidized and reduced states. Fe K-edge data mainly shows that the atypical metal coordination geometry of cytochrome f, in which the N-terminal amino...
Uploaded on: December 4, 2022 -
October 17, 2023 (v1)Publication
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Uploaded on: October 18, 2023 -
April 7, 2022 (v1)Publication
More than 50 years have passed since Nobel laureate Cristian de Duve described for the first time the presence of tiny subcellular compartments filled with hydrolytic enzymes: the lysosome. For a long time, lysosomes were deemed simple waste bags exerting a plethora of hydrolytic activities involved in the recycling of biopolymers, and...
Uploaded on: March 25, 2023 -
March 6, 2018 (v1)Publication
Human antigen R (HuR) is a multitasking RNA binding protein involved in posttranscriptional regulation by recognizing adenine- and uracile-rich elements placed at the 3′-untranslated regions of messenger RNAs (mRNAs). The modular architecture of the protein, which consists of two N-terminal RNA recognition motifs (RRMs) in tandem spaced from a...
Uploaded on: December 4, 2022 -
September 14, 2021 (v1)Publication
While most organelles are surrounded by membranes, cells also contain membraneless organelles, which remain separated in the cell by avoiding the mixture of their components with the surroundings. Actually, liquid–liquid phase separation provides a simple but smart mechanism for the cell to control the spatial localization and processing of...
Uploaded on: December 4, 2022 -
February 21, 2018 (v1)Publication
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Uploaded on: March 27, 2023 -
May 26, 2022 (v1)Publication
The complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc has been characterized by NMR spectroscopy. The binding constant is 16 mm–1, and the lifetime of the complex is much less than 10 ms. Intermolecular pseudo-contact shifts observed for the plastocyanin amide nuclei, caused by the heme iron, as well as the...
Uploaded on: March 25, 2023 -
January 23, 2019 (v1)Publication
BACKGROUND: Even though liver kinase B1 (LKB1) is usually described as a tumor suppressor in a wide variety of tissues, it has been shown that LKB1 aberrant expression is associated with bad prognosis in Hepatocellular Carcinoma (HCC). METHODS: Herein we have overexpressed LKB1 in human hepatoma cells and by using histidine pull-down assay we...
Uploaded on: March 27, 2023 -
May 26, 2022 (v1)Publication
Transient complexes, with a lifetime ranging between microseconds and seconds, are essential forbiochemical reactions requiring a fast turnover. That is the case of the interactions between proteinsengaged in electron transfer reactions, which are involved in relevant physiological processes suchas respiration and photosynthesis. In the latter,...
Uploaded on: March 25, 2023 -
March 27, 2019 (v1)Publication
The metal cofactor determines the thermal stability in cupredoxins, but how the redox state of copper modulates their melting points remains unknown. The metal coordination environment is highly conserved in cyanobacterial plastocyanins. However, the oxidised form is more stable than the reduced one in thermophilic Phormidium, but the opposite...
Uploaded on: December 5, 2022 -
June 1, 2017 (v1)Publication
TIA-1 (T-cell restricted intracellular antigen-1) is an RNA-binding protein involved in splicing and translational repression. It mainly interacts with RNA via its second and third RNA recognition motifs (RRMs), with specificity for U-rich sequences directed by RRM2. It has recently been shown that RRM3 also contributes to binding, with...
Uploaded on: March 25, 2023