DIASPRO , Alberto

Taking inspiration from the philosophical and sociological speculation by Zygmunt Bauman (Z. Bauman, Liquid Modernity, Polity Press, Cambridge, 2000) a new paradigm for optical microscopy is proposed regarding design, implementation, and applications. The two-photon excitation microscope of the future, combining different converging...

The advent of two-photon excitation (2PE) to produce microscopy images is the most important revolution in fluorescence microscopy. Since the recording of fluorescence by Sheppard within a non linear scanning microscopy scheme in 1978 to the effective application in 1990 by Denk and colleagues at the Webb labs at Cornell, 2PE microscopy changed...

Multiphoton microscopy was more than a revolution in optical microscopy and more in general in optical bioimaging. In the last 25 years has enabled unprecedented exploration of biological systems including living organisms. Two-photon excitation of fluorescence is only one of the aspects that made multiphoton microscopy a key method for...

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This chapter discusses in detail the current problems related to the preparation of liquid films in laser-induced forward transfer (LIFT), from fundamental aspects to practical problems. It introduces film-free LIFT (FF-LIFT), explains its physical principles, and describes the different modalities in which the technique can be implemented. It...