DIASPRO A.

Since cells and tissues are inherently three-dimensional (3D), 3D imaging techniques are required to study them. 3D light microscopy offers a noninvasive, minimally destructive option for obtaining spatial and volumetric information about the structure and function of cells and tissues. 3D image data acquired with confocal or multiphoton...

After publication of the article we noticed that equations number 24 and 25 missed a convolution sign.

Circular Intensity Differential Scattering (CIDS) provides a differential measurement of the circular right and left polarized light and has been proven to be a gold standard label-free technique to study the molecular conformation of complex biopolymers, such as chromatin. In early works, it has been shown that the scattering component of the...

We propose a methodology for the study of protein-DNA electrostatic interactions and apply it to clarify the effect of histone tails in nucleosomes. This method can be used to correlate electrostatic interactions to structural and functional features of protein-DNA systems, and can be combined with coarse-grained representations. In particular,...

Understanding the structural organization of chromatin is essential to comprehend the gene functions. The chromatin organization changes in the cell cycle, and it conforms to various compaction levels. We investigated a chromatin solenoid model with nucleosomes shaped as cylindrical units arranged in a helical array. The solenoid with...

The high versatility of laser direct-write (LDW) systems offers remarkable opportunities for Industry 4.0. However, the inherent serial nature of LDW systems can seriously constrain manufacturing throughput and, consequently, the industrial scalability of this technology. Here we present a method to parallelise LDWs by using acoustically shaped...

A novel tool for the simultaneous shaping and parallelization of a laser beam is presented. Our method exploits the interaction of light and ultrasound in a fluid to form various light intensity patterns with a large tunability and at high speed. Arrays of tailored beams (Gaussian, annular, or Bessel) can be formed by properly selecting the...

DEK protein, a key chromatin regulator, is strongly overexpressed in various forms of cancer. While conventional microscopy revealed DEK as uniformly distributed within the cell nucleus, advanced super-resolution techniques uncovered cluster-like structures. However, a comprehensive understanding of DEK's cellular distribution and its...

Optical scanning microscopy techniques based on the polarization control of the light have the capability of providing non invasive label-free contrast. By comparing the polarization states of the excitation light with its transformation after interaction with the sample, the full optical properties can be summarized in a single 4  4 Mueller...

We demonstrate a combination of polarization-resolved with fluorescence optical scanning microscopy that offers polarizing based information along with molecular view of sample as a versatile tool for imaging the chromatin organization.