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DRAKE GARCÍA , Rocío

Last name: DRAKE GARCÍA

First name: Rocío

April 5, 2019 (v1)

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N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast

Drake García, Rocío Serrano Delgado, Aurelio Pérez Castiñeira, José Román

Expression of heterologous multispanning membrane proteins in Saccharomyces cerevisiae is a difficult task. Quite often, the use of multicopy plasmids where the foreign gene is under the control of a strong promoter does not guarantee efficient production of the corresponding protein. In the present study, we show that the expression level...

Uploaded on: March 27, 2023
February 27, 2015 (v1)

Publication

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A plant proton-pumping inorganic pyrophosphatase functionally complements the vacuolar ATPase transport activity and confers bafilomycin resistance in yeast

Drake García, Rocío Serrano Delgado, Aurelio Pérez Castiñeira, José Román

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Uploaded on: March 27, 2023