DULIC , Vjekoslav

Human diploid fibroblasts have a finite proliferative lifespan in culture, at the end of which they are arrested with G1 phase DNA contents. Upon serum stimulation, senescent cells are deficient in carrying out a subset of early signal transduction events such as activation of protein kinase C and induction of c-fos. Later in G1, they uniformly...

ABSTRACT Cell cycle arrest in G 1 in response to ionizing radiation or senescence is believed to be provoked by inactivation of G 1 cyclin-cyclin-dependent kinases (Cdks) by the Cdk inhibitor p21 Cip1/Waf1/Sdi1 . We provide evidence that in addition to exerting negative control of the G 1 /S phase transition, p21 may play a role at the onset of...

Tissue homeostasis requires precise control of cell proliferation and arrest in response to environmental cues. In situation such as wound healing, injured cells are stimulated to divide, but as soon as confluence is reached proliferation must be blocked. Such reversible cell cycle exit occurs in G(1), requires pRb family members, and is driven...

ABSTRACT Bcl-x L , a member of the Bcl-2 family, inhibits apoptosis, and its expression is regulated at the transcriptional level, yet nothing is known about the transcription factors specifically activating this promoter. The bcl-x promoter contains potential Ets binding sites, and we show that the transcription factor, Ets2, first identified...