FRUTOS BELTRÁN , Estrella

ESTRUCTURA Y ESTABILIDAD EN PLASTOCIANINAS DE CIANOBACTERIAS La elucidación de los factores que determinan las diferencias en la termorresistencia en las proteínas de organismos termófilos respecto a sus homólogas de organismos mesófilos es un tema de elevado interés en relación a la ingeniería de proteínas. Cada familia proteica adopta...

Many fleeting macromolecular interactions, like those being involved in electron transport, are essential in biology. However, little is known about the behaviour of the partners and their dynamics within their shortlived complex. To tackle such issue, we have performed molecular dynamics simulations on an electron transfer complex formed by...

The metal cofactor determines the thermal stability in cupredoxins, but how the redox state of copper modulates their melting points remains unknown. The metal coordination environment is highly conserved in cyanobacterial plastocyanins. However, the oxidised form is more stable than the reduced one in thermophilic Phormidium, but the opposite...

Redox thermodynamics and kinetics of plastocyanin from Phormidium laminosum, and of azurin from Pseudonomas aeruginosa, have been investigated as a function of temperature by protein film voltammetry. To this purpose, both proteins have been physisorbed on a pyrolytic graphite edge electrode. A pronounced negative shift of the plastocyanin...

Understanding the molecular basis of the thermal stability and functionality of redox proteins has important practical applications. Here, we show a distinct thermal dependence of the spectroscopic and electrochemical properties of two plastocyanins from the thermophilic cyanobacterium Phormidium laminosum and their mesophilic counterpart from...