GORGUN , Cansu

Mesenchymal Stromal Cells (MSCs) are currently considered a powerful tool for the treatment of multiple diseases. Indeed, the original hypothesis underlying MSC based regenerative therapies was based on functional recovery as a consequence of stem cell differentiation. But it is now clear that other mechanisms of action are involved. The recent...

This unit describes protocols for isolating subpopulations of extracellular vesicles (EVs) purified from human adipose tissue–derived mesenchymal stromal cells by density gradient centrifugation and for characterizing them by flow cytometry (FCM). Determining the optimal strategy for isolating EVs is a critical step toward retrieving the...