GAMBALE , Franco

Poyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Valcuolar (SV) channel encoded by the plant TPC1 gene. The patchclamp technique was applied to isolated vaculoes from carrot taproots and Arabidopsis thaliana mesophyll cells and...

We investigated the biophysical properties of the transport mediated by ion channels in hemocytes from the hemolymph of the bivalve Mytilus galloprovincialis. Besides other transporters, mytilus hemocytes possess a specialized channel sensitive to the osmotic pressure with functional properties similar to those of other transport proteins...

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Our research introduces the natural flavonoid naringenin as a novel inhibitor of an emerging class of intracellular channels, Two-Pore Channel 2 (TPC2), as shown by electrophysiological evidence in a heterologous system, i.e. Arabidopsis vacuoles lacking endogenous TPCs. In view of the control exerted by TPC2 on intracellular calcium signaling,...

• Ion transport proteins in intracellular membranes of eukaryotic cells play key roles in many physiological and pathological processes. • The function of many of these transporters is poorly understood, because their intracellular localization makes them difficult to study. • Here, we used the large organelle of plant cells, the central...