En esta Tesis se han utilizado mutantes fotorrespiratorios de Lotus japonicus deficientes en la enzima glutamina sintetasa plastidica para examinar la funcionalidad de esta enzima, analizando las consecuencias fisiológicas de esta mutación a distintos niv
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November 27, 2014 (v1)PublicationUploaded on: December 4, 2022
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February 26, 2024 (v1)Publication
Photorespiration has been considered a 'futile' cycle in C3 plants, necessary to detoxify and recycle the metabolites generated by the oxygenating activity of Rubisco. However, several reports indicate that this metabolic route plays a fundamental role in plant metabolism and constitutes a very interesting research topic. Many open questions...
Uploaded on: February 28, 2024 -
March 16, 2017 (v1)Publication
It is well established that the plastidic isoform of glutamine synthetase (GS2) is the enzyme in charge of photorespiratory ammonium reassimilation in plants. The metabolic events associated to photorespiratory NH4+ accumulation were analyzed in a Lotus japonicus photorespiratory mutant lacking GS2. The mutant plants accumulated high levels of...
Uploaded on: March 27, 2023 -
December 15, 2020 (v1)Publication
Phenylpropanoid metabolism represents an important metabolic pathway from which originates a wide number of secondary metabolites derived from phenylalanine or tyrosine, such as flavonoids and isoflavonoids, crucial molecules in plants implicated in a large number of biological processes. Therefore, various types of interconnection exist...
Uploaded on: March 26, 2023 -
February 23, 2024 (v1)Publication
Isoflavonoids are mostly produced by legumes although little is known about why and how legumes are able to regulate the biosynthesis of these particular compounds. Understanding the role of potential regulatory genes of the isoflavonoid biosynthetic pathway constitutes an important topic of research. The LORE1 mutation of the gene encoding the...
Uploaded on: February 25, 2024 -
June 15, 2021 (v1)Publication
: Several aspects of the legume–rhizobia symbiosis are far from being completely understood, such as the transport of compounds through the symbiosome membrane and the molecular actors (receptors, transcription factors and hormones) involved in the systemic regulation of nodulation. In this work, the transcriptomes of L. japonicus plants...
Uploaded on: March 27, 2023 -
February 27, 2015 (v1)Publication
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Uploaded on: December 4, 2022 -
January 10, 2019 (v1)Publication
Background: Asparagine is a very important nitrogen transport and storage compound in plants due to its high nitrogen/carbon ratio and stability. Asparagine intracellu lar concentration depends on a balance between asparagine biosynthesis and degradation. The main enzymes involved in asparagine metabolism are as paragine synthetase...
Uploaded on: March 27, 2023 -
June 10, 2020 (v1)Publication
Gene therapy is a therapeutic process consisting of the transport of genetic material into cells. The design and preparation of novel carriers to transport DNA is an important research line in the medical field. Hybrid compounds such as metallo-liposomes, containing a mixture of lipids, were prepared and characterized. Cationic metal lipids...
Uploaded on: March 25, 2023 -
April 28, 2017 (v1)Publication
Nitrogen is one of the most important nutrients for plants and, in natural soils, its availability is often a major limiting factor for plant growth. Here we examine the effect of different forms of nitrogen nutrition and of photorespiration on gene expression in the model legume Lotus japonicus with the aim of identifying regulatory candidate...
Uploaded on: December 4, 2022 -
July 8, 2021 (v1)Publication
The interaction between calf-thymus DNA, ctDNA, and various single-chained surfactants with different functional groups at the end of hydrophobic tail was studied with the goal of investigating the influence of the functional group nature on surfactant DNA compacting efficiency. The surfactants investigated were dodecyltriethylammonium bromide...
Uploaded on: March 25, 2023 -
July 19, 2023 (v1)Publication
Hydrogen sulfide is a signaling molecule in plants that regulates essential biological processes through protein persulfidation. However, little is known about sulfide-mediated regulation in relation to photorespiration. Here, we performed label-free quantitative proteomic analysis and observed a high impact on protein persulfidation levels...
Uploaded on: October 18, 2023