GHIGGERI G. M.

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Molecular biology has developed between years 1940s and 1960s as a branch of science that spans from genetics to cell biology and biochemistry with the goal of understanding the interactions by which DNA, RNA, and protein synthesis operate into cells [1]. Since then, numerous technological innovations pushed the field forward, allowing...

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Ultracentrifugationon sucrose density gradientappears to be the best purification protocol for extracellular vesicle (EVs) purification. After this step, to reduce disulfide bridges linking exogenous proteins to the vesicles, the collected samples are routinely washed and treated with dithiothreitol (DTT). Such incubations are performed at...