GUIGNARD , Léo

Image-based studies of developing organs or embryos produce a huge quantity of data. To handle such high-throughput experimental protocols, automated computer-assisted methods are highly desirable. This article aims at designing an efficient cell segmentation method from microscopic images. The proposed approach is twofold: first, cell...

Image-based studies of developing organs or embryos produce a huge quantity of data. To handle such high-throughput experimental protocols, automated computer-assisted methods are highly desirable. This article aims at designing an efficient cell segmentation method from microscopic images. The proposed approach is twofold: first, cell...

Current imaging techniques can capture temporal sequences of 3D images with very high time resolution over several hours. Com- paring sequences covering the same time period opens the way to the study of developmental variability. Stitching together sequences captured from different embryos may help producing a sequence covering the whole...

Recent microscopy techniques allow imaging temporal 3D stacks of developing organs or embryos with a cellular level of resolution and with a sufficient acquisition frequency to accurately track cell lineages. Imaging multiple organs or embryos in different experimental conditions may help decipher the impact of genetic backgrounds and...

Current imaging techniques can capture temporal sequences of 3D images with very high time resolution over several hours. Com- paring sequences covering the same time period opens the way to the study of developmental variability. Stitching together sequences captured from different embryos may help producing a sequence covering the whole...

The mouse embryo has long been central to the study of mammalian development; however, elucidating the cell behaviors governing gastrulation and the formation of tissues and organs remains a fundamental challenge. A major obstacle is the lack of live imaging and image analysis technologies capable of systematically following cellular dynamics...

Canalization of developmental processes ensures the reproducibility and robustness of embryogenesis within each species. In its extreme form, found in ascidians, early embryonic cell lineages are invariant between embryos within and between species, despite rapid genomic divergence. To resolve this paradox, we used live light-sheet imaging...

Marine invertebrate ascidians display embryonic reproducibility: Their early embryonic cell lineages are considered invariant and are conserved between distantly related species, despite rapid genomic divergence. Here, we address the drivers of this reproducibility. We used light-sheet imaging and automated cell segmentation and tracking...