Using Helix pomatia lectin as a specific probe for terminal, nonreducing N-acetylgalactosamine residues, glycoprotein precursors bearing newly initiated O-linked oligosaccharides have been localized in the lumen of the endoplasmic reticulum and cis-Golgi cisternae. This pattern contrasts with the detection of the terminal disaccharide galactose...
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May 3, 2016 (v1)PublicationUploaded on: December 4, 2022
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April 18, 2016 (v1)Publication
Streptolysin O-permeabilized cells incubated with a high concentration (5-10 mg/ml) of cytosolic proteins and ATPgenerating system exhibit redistribution into the endoplasmic reticulum (ER) of Golgi integral proteins (mannosidase II, galactosyltransferase, TGN 38), detected by immunofluorescence. In addition, mannosidase II is detected in...
Uploaded on: March 27, 2023 -
March 3, 2015 (v1)Publication
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Uploaded on: March 27, 2023 -
May 3, 2016 (v1)Publication
The influence of protein kinase A activity on transport of newly synthesized vesicular stomatitis virus G glycoprotein along the exocytic pathway was examined. Transport of vesicular stomatitis virus G glycoprotein to the cell surface was inhibited by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), a selective inhibitor of...
Uploaded on: December 5, 2022 -
February 26, 2016 (v1)Publication
Ultracytochemical analysis of acid phosphatase in the nephrocytes of Helix aspersa renal sac was carried out using beta-glycerophosphate as the substrate of reaction. The reaction product was localized on dictyosomes, lysosomes, apical and lateral plasma membranes and, occasionally, in dense digestive bodies. In the latter, the lack of reaction...
Uploaded on: December 4, 2022 -
April 27, 2016 (v1)Publication
We have examined the role played by protein kinase A (PKA) in vesicle-mediated protein transport from the trans-Golgi network (TGN) to the cell surface. In vivo this transport step was inhibited by inhibitors of PKA catalytic subunits (C-PKA) such as the compound known as H89 and a myristoylated form of the inhibitory peptide sequence contained...
Uploaded on: December 4, 2022 -
April 26, 2016 (v1)Publication
The Golgi complex represents a major subcellular location of protein kinase A (PKA) concentration in mammalian cells where it has been previously shown to be involved in vesicle-mediated protein transport processes. We have studied the factors that influence the interaction of PKA typeII subunits with the Golgi complex. In addition to the...
Uploaded on: December 4, 2022 -
April 26, 2016 (v1)Publication
The mammalian Golgi complex consists of stacks of cisternae linked laterally into a continuous perinuclear ribbon structure. Protein kinase A is stably associated with the Golgi complex during interphase. To analyze its role in Golgi structural maintenance cells were depleted of protein kinase A regulatory subunits using small interfering RNAs....
Uploaded on: December 4, 2022 -
May 3, 2016 (v1)Publication
The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes the formation of membranebound transport intermediates. By using an in vitro assay that reproduces recruitment of cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed the role of...
Uploaded on: December 4, 2022 -
April 18, 2016 (v1)Publication
The KDEL receptor is a Golgi/intermediate compartment-located integral membrane protein that carries out the retrieval of escaped ER proteins bearing a C-terminal KDEL sequence. This occurs throughout retrograde traffic mediated by COPI-coated transport carriers. The role of the C-terminal cytoplasmic domain of the KDEL receptor in this process...
Uploaded on: March 27, 2023