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Last name: JAUBERT-POSSAMAI
First name: Stéphanie
National audience
"Cet ouvrage très didactique présente, sous forme de fiches, les principales techniques utilisées dans les laboratoires pour étudier le fonctionnement du vivant via les acides nucléiques. Après quelques définitions et généralités sur la structure des gènes, on y décrit les techniques de base en biologie moléculaire et génomique. Ces techniques...
Plant-parasitic root-knot and cyst nematodes are microscopic worms that cause severe damage to crops and induce major agricultural losses worldwide. These parasites penetrate into host roots and induce the formation of specialized feeding structures, which supply the resources required for nematode development. Root-knot nematodes induce the...
Among plant-parasitic nematodes, the root-knot nematodes (RKNs) of the Meloidogyne spp. are the most economically important genus. RKN are root parasitic worms able to infect nearly all crop species and have a wide geographic distribution. During infection, RKNs establish and maintain an intimate relationship with the host plant. This includes...
The spatio-temporal expression pattern of a gene provides important indications to betterunderstand its biological function. In situ hybridization (ISH) uses a labeled complementarysingle-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or asection of tissue. We adapted the ISH technique to the plant...
Root-knot nematodes (RKNs) are root endoparasites that induce the dedifferentiation of a few root cells and the reprogramming of their gene expression to generate giant hypermetabolic feeding cells.We identified two microRNA families, miR408 and miR398, as upregulated in Arabidopsis thaliana and Solanum lycopersicum roots infected by RKNs. In...
BackgroundRoot-knot nematodes (RKN), genus Meloidogyne, are plant parasitic worms that have the ability to transform root vascular cylinder cells into hypertrophied, multinucleate and metabolically over-active feeding cells. Redifferentiation into feeding cells is the result of a massive transcriptional reprogramming of root cells targeted by...
Root-knot nematodes are obligate endoparasites that maintain a biotrophic relationship with their hosts over a period of several weeks. They induce the differentiation of root cells into specialized multinucleate hypertrophied feeding cells known as giant cells. Nematode effectors synthesized in the esophageal glands and injected into the plant...
Abstract Root-knot nematodes (RKNs) are among the most damaging pests of agricultural crops. Meloidogyne is an extremely polyphagous genus of nematodes that can infect thousands of plant species. A few genes for resistance (R-genes) to RKN suitable for use in crop breeding have been identified, but virulent strains and species of RKN have...
Phenotypic plasticity is an adaptive process widespread in Insects. This involves the capacity of embryos to sense external local environmental cues during their development with alternative routes providing a final phenotype adapted to those new cues. In our lab, we describe and analyse the gene expression regulation rules during the...
Root knot nematodes (RKN) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. MicroRNAs (miRNAs) regulate gene expression during development and plant responses to various stresses.Disruption of post-transcriptional gene silencing in Arabidopsis ago1 or ago2...