KOROBCHEVSKAYA , Kseniya

Although modern optical microscopy allows the achievement of sub-diffraction resolution, most of the current techniques rely on a fluorescence contrast mechanism. Moreover, deep tissue imaging remains a challenging task especially for thick and highly scattering biological objects. The infrared absorption/saturation microscopy method is...

At present, most optical microscopy techniques provide sub-diffraction scale imaging based on fluorescence as the underlying contrast mechanism. Fluorescence introduces certain limitations such as a reliance on labels, photo-bleaching and a reduction of penetration depth. Two-photon excitation microscopy, which utilizes near IR femtosecond...

We propose and demonstrate a novel subtraction microscopy algorithm, exploiting fluorescence emission difference or switching laser mode and their derivatives for image enhancement. The key novelty of the proposed approach lies in the weighted subtraction coefficient, adjusted pixel-by-pixel with respect to the intensity distributions of...

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