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KOTRBA , Pavel

Last name: KOTRBA

First name: Pavel

June 7, 2016 (v1)

Publication

Metadata-only

Metalloadsorption by Escherichia coli cells displaying yeast and mammalian metallothioneins anchored to the outer membrane protein LamB

Sousa Martín, Carolina Kotrba, Pavel Ruml, Tomáš

Yeast (CUP1) and mammalian (HMT-1A) metallothioneins (MTs) have been efficiently expressed in Escherichia coli as fusions to the outer membrane protein LamB. A 65-amino-acid sequence from the CUP1 protein of Saccharomyces cerevisiae (yeast [Y] MT) was genetically inserted in permissive site 153 of the LamB sequence, which faces the outer...

Uploaded on: March 27, 2023