LÓPEZ CALDERÓN , Isabel

Se ha estudiado el efecto del Mutageno n-metil-n-nitro-n-nitrosoguanidina en la levadura Saccharomyces cerevisiae. Se ha determinado cuales son las condiciones a las que se obtiene una frecuencia de mutantes máxima. No se ha encontrado comutación entre los genes his4a his4b e his4c ni entre los genes cdc15 y ade1 situados muy próximos entre si....

Lección inaugural del Aula de la Experiencia del curso 2017/2018 en el que la autora se decanta por tratar el tema de la biología de la determinación sexual en humanos, porque es un asunto que está en la calle y que suscita polémicas muy enconadas, sostenidas la mayor parte de las veces por argumentos muy emocionales pero poco científicos. En...

Production of fl-carotene by wild-type Phycomyces blakesleeanus can be stimulated by light, chemicals, regulatory mutations, and sexual interaction between mycelia of opposite sex. Through genetic manipulations, we have isolated strains which have simultaneously and constitutively incorporated several of these stimulatory effects. In the dark...

Excretion of amino acids by yeast cells was reported long ago but has not been characterized in molecular terms. It is typically favored by overproduction of the amino acid and/or impairment of its uptake. Here we describe the construction of a yeast strain excreting threonine and homoserine. Using this excretor strain, we then applied a...

To obtain strains that are able to efficiently produce ethanol from different carbohydrates, mainly cellulose hydrolysates, several species of the genus Candida and a Zygosaccharomyces fermentati strain were examined for their ability to utilize cellobiose and produce ethanol, as well as for their thermotolerance and the possibility of genetic m...

In this work, we isolated and characterized mutants that overproduce threonine from Saccharomyces cerevisiae. The mutants were selected for resistance to the threonine analog a-amino-13-hydroxynorvalerate (hydroxynorvaline), and, of these, the ones able to excrete threonine to the medium were chosen. The mutant strains produce between 15 and 30 ...

The HOM3 gene of Saccharomyces cerevisiae codes for aspartate kinase, which plays a crucial role in the regulation of the metabolic flux that leads to threonine biosynthesis. With the aim of obtaining yeast strains able to overproduce threonine in a controlled way, we have placed the HOM3-R2 mutant allele, which causes expression of...

Representatives of Arthrocnemum, Salicornia and Sarcocornia were analysed using RAPO. The methods used were optimized by testing different conditions of the parameters. The genetic characterization of the mentioned taxa proved possible, demonstrating the usefulness of RAPO techniques to determine generic limits in the Salicomieae