2009 (v1)
Publication
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2013 (v1)Publication
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2009 (v1)Publication
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2013 (v1)Publication
In this work we report the advantages provided by two photon excitation (2PE) implemented in a selective plane illumination microscopy (SPIM) when imaging thick scattering samples. In particular, a detailed analysis of the effects induced on the real light sheet excitation intensity distribution is performed. The comparison between...
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2010 (v1)Publication
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2013 (v1)Publication
Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers...
Uploaded on: March 27, 2023 -
2013 (v1)Publication
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2011 (v1)Publication
Light-sheet microscopy, such as ultramicroscopy, single plane illumination microscopy (SPIM) [1] and digital scanned laser microscopy (DSLM) [2], represents a useful tool for biological investigations of thick samples. Such techniques have been found particularly useful in developmental biology applications since they provide the capability to...
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2010 (v1)Publication
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2016 (v1)Publication
In the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could...
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2013 (v1)Publication
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Uploaded on: April 14, 2023