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Last name: LEFEBVRE
First name: Véronique
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Potato cyst nematodes induce changes in plant host gene expression following root invasion. For an accurate comparison of gene expression by reverse transcription quantitative real-time PCR (RT-qPCR), internal reference genes are necessary for transcript normalization. Very few experimental data on suitable reference genes are available for...
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Among Solanaceous crops, pepper and tomato are damaged by numerous pathogens, including Phytophthora capsici and Phytophthora infestans. Major genes have been identified in tomato to confer resistance against P. infestans, which were rapidly overcome by virulent populations. Pepper resistance towards P. capsici is provided by several...
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Oomycetes form a diverse group of fungus-like eukaryotic microorganisms that include saprophytes as weil as pathogens of animais and some of the most devastating pathogens of dicotyledonous plants. Among them, few species of the genus Phytophthora, such as P. capsici and P. infestons cause enormous economic da mage on Solanaceous crops. Major...
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Potato cyst nematodes induce changes in gene expression after infecting the roots. For studying gene expression, reverse transcription quantitative real-time PCR (RT-qPCR) is now a standard method. However, for accurate quantication of transcripts, a set of internal control genes, stable under different experimental conditions, is necessary for...
The PM687 line of Capsicum annuum L. has a single dominant gene, Me-3, that confers heat-stable resistance to root-knot nematodes (RKN). Me-3 was mapped using doubled-haploid (DH) lines and F-2 progeny from a cross between the susceptible cultivar 'Yolo Wonder' ('YW') and the highly resistant line 'PM687'. Bulked-segregant analysis with DNA...
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