MUSSINO , Francesca Maria

Sponge cells represent a unique model for the study of the molecular evolution of animal metabolism and of the ancestral processes of biomineralization. Among various sponge culture models, the most promising ones are based on the primmorph technique, which allows the production of 3D sponge cell aggregates, easily reared in a laboratory...

The possibility to cryopreserve cells allows for wide opportunities of flexible handling of cell cultures from different sponge species. Primmorphs model, a multicellular 3D aggregate formed by dissociated sponge cells, is considered one of the best approaches to establish sponge cell culture but, in spite of the available protocols for...

Prolyl 4-hydroxylase (P4H) catalyzes the hydroxylation of proline residues in collagen. P4H has two functional subunits, α and β. Here, we report the cDNA cloning, characterization, and expression analysis of the α and β subunits of the P4H derived from the marine sponge Chondrosia reniformis. The amino acid sequence of the α subunit is 533...

Here we report the molecular cloning and characterization of the first Tumor Necrosis Factor homologous and of its putative receptor in the marine sponge Chondrosia reniformis: chTNF and chTNFR, respectively. The deduced chTNF amino acid sequence is a type II transmembrane protein containing the typical TNFSF domain. Phylogenetic analysis...

Prolyl 4-hydroxylase (P4H) is a alpha-2 beta-2 tetramer catalyzing the post-translational hydroxylation of prolinesin collagen. Its recombinant production is mainly pursued to realize biotechnological tools able to gener-ate animal contaminant-free hydroxylated collagen. One promising candidate for biomedical applicationsis the collagen...

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