MORENO BELTRÁN , José Blas

Protein complex formation is at least a two-step process in which the formation of a final, well-defined complex entails the initial formation of a dynamic encounter complex. Highly transient complexes, with lifetimes in the order of microseconds-milliseconds, exhibit moderate or low binding affinities, with dissociation constants in the...

n plants, channeling of cytochrome c molecules between complexes III and IV has been purported to shuttle electrons within the supercomplexes instead of carrying electrons by random diffusion across the intermembrane bulk phase. However, the mode plant cytochrome c behaves inside a supercomplex such as the respirasome, formed by complexes I,...

Electron-transfer kinetics of the thermophilic protein Plastocyanin fromPhormidium laminosumadsorbed on 1,ω-alkanedithiol self-assembled monolayers (SAMs)deposited on gold have been investigated. The standard electron-transfer rate constant hasbeen determined as a function of electrode−protein distance and solution viscosity over abroad...

The transient interactions of respiratory cytochrome c with complexes III and IV is herein investigated by using heterologous proteins, namely human cytochrome c, the soluble domain of plant cytochrome c1 and bovine cytochrome c oxidase. The binding molecular mechanisms of the resulting cross-complexes have been analyzed by Nuclear Magnetic...

Chromatin is pivotal for regulation of the DNA damage process insofar as it influences access to DNA and serves as a DNA repair docking site. Recent works identify histone chaperones as key regulators of damaged chromatin's transcriptional activity. However, understanding how chaperones are modulated during DNA damage response is still...

Regulation of mitochondrial activity allows cells to adapt to changing conditions and to control oxidative stress, and its dysfunction can lead to hypoxia-dependent pathologies such as ischemia and cancer. Although cytochrome c phosphorylation—in particular, at tyrosine 48—is a key modulator of mitochondrial signaling, its action and molecular...

l‐galactono‐1,4‐lactone dehydrogenase (GALDH) catalyzes the terminal step of vitamin C biosynthesis in plant mitochondria. Here we investigated the communication between Arabidopsis thaliana GALDH and its natural electron acceptor cytochrome c (Cc). Using laser‐generated radicals we observed the formation and stabilization of the GALDH...

The rapid transfer of electrons in the photosynthetic redox chain is achieved by the formation of short-lived complexes of cytochrome b6f with the electron transfer proteins plastocyanin and cytochrome c6. A balance must exist between fast intermolecular electron transfer and rapid dissociation, which requires the formation of a complex that...