MORENO ONORATO , Francisco Javier

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Programmed cell death (PCD) is a crucial phenomenon in the life cycle of cereal grains. In germinating grains, the scutellum allows the transport of nutrients from the starchy endosperm to the growing embryo, and therefore it may be the last grain tissue to undergo PCD. Thus, the aim of this work was to analyse whether the scutellum of wheat...

The aleurone layer of cereal grains undergoes a gibberellin-regulated process of programmed cell death (PCD) following germination. We have applied a combination of ultrastructural and biochemical approaches to analyze aleurone PCD in intact wheat grains. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay revealed...

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Para comprobar la acción de la pirenzepina sobre células parietales en regeneración, hemos realizado un estudio estereológico y ultraestructural. Para ello utilizamos grupos controles y de tratamiento con el fármaco a dosis de 1, 5 y 25 mg/kg/día, operados y no operados. En los grupos operados se realizó una úlcera por cauterización mediante...

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Para comprobar la acción de la ranitidina sobre células parietales en regeneración hemos realizado un estudio morfológico y estereológico. Para ello utilizamos grupos controles y de tratamiento con el fármaco a dosis de dos, 10 y 50 mg!kg/día, operados y no operados. En los grupos operados se realizó una úlcera por cauterización mediante...

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Purified polyclonal antibodies have been obtained against 100 KD and 36 KD Ag-NOR proteins. These two antibodies have been used to study the immunolocalization of both proteins on different cell types subjected to various conditions of cell activity and to observe a nucleolar labelling with anti-36 kDa Ag-NOR protein antibody evenly distributed...

A trans-diaquacomplex formed by copper(II) sulphate and the sequestering polyamminopolycarboxylic ligand 1,2-propylenediaminetetraacetic acid (PDTA) has been isolated and characterized by chemical analysis, titrimetry, FT-IR and electronic spectroscopy, Potentiometric and electronic measurements identified the ligand as tetradentate, two...

Morphological modifications and cytochemical changes in the acid phosphatase on oxintic cells of the gastric mucosa of rats deprived of food for varying lengths of time were studied. With long fasting periods a lysosomal qualitative increase and mitochondrial morphological alteration are caused. This last fact would make an ATP decrease and an...

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The susceptibility of different tissues and organs from strawberry plants, cv "Camarosa", to Colletotrichum acutatum was tested using a severity index based on infection response. Symptoms developed on inoculated tissues were characterized along 30 days. Flowers, except sepals, petioles and fruits were the most susceptible organs to the...

The interaction of a newly synthesized antitumor complex cis-dichloro-1,2-propylenediamine-N,N,N',N'-tetraacetato ruthenium (III) (RAP) with DNA was investigated in vitro through a number of techniques including comet assay, immunoprecipitation, and immunolocalization of certain nucleolar proteins (the upstream binding factor (UBF) and...