MUÑOZ PINTO , Mario Faustino

The present thesis is a compilation of several experiments about the use of adiposederived mesenchymal stem cells (ADSCs) in cell therapy. The study focus on the behavior, molecular mechanisms and environment changes of these cells during aging and under oxidative stress, that could determine its efficacy in Regenerative Medicine. Adipose...

Substance P (SP) is a neuropeptide belonging to the thachykinin peptide family. SP, after binding to its receptor, the neurokinin 1 receptor (NK1R), controls several transcription factors such as NF-κB, hypoxia inducible factor (HIF-1α), c-myc, c-fos, c-jun, and AP-1. SP and NK1R have a widespread distribution in both the central and peripheral...

Ageing is characterised by the accumulation of molecular and cellular damage through time, leading to a decline in physical and mental abilities. Currently, society has experienced a rapid increase in life expectancy, which has led to an increase in age-associated diseases. Therefore, it is crucial to study the process of ageing to guarantee...

Lipid peroxidation can be described generally as a process under which oxidants such as free radicals attack lipids containing carbon-carbon double bond(s), especially polyunsaturated fatty acids (PUFAs). Over the last four decades, an extensive body of literature regarding lipid peroxidation has shown its important role in cell biology and...

Since 1956, when Harman first postulated the free radical theory of aging, numerous studies have been carried out to test the protective action of antioxidants. One of these protective compounds used in antioxidant therapy is folic acid (FA). Folate deficiency can lead to several pathologies and its protective role is very well known. Because...

Background. Glioblastoma or glioma is the most common malignant brain tumor. Patients have a prognosis of approximately 15 months, despite the current aggressive treatment. Neurokinin-1 receptor (NK-1R) occurs naturally in human glioma, and it is necessary for the tumor development. Objective. The purpose of the study was to increase the...

We studied the alterations of Elongation Factor 2 (eEF2) in the pineal gland of aged rats as well as the possible protective role of exogenous melatonin on these changes in young rats treated with cumene hydroperoxide (CH), a compound that promotes lipid peroxidation and inhibits protein synthesis. The study was performed using male Wistar rats...

Researchers cannot predict as yet how long a human being can live. Life expectancy has been steadily increasing in the last century, but perhaps not always the quality of life in parallel with it. Future generations will be faced with the problems of an increased life expectancy along with the emergence of new age-related diseases. A deeper...

As organisms age, a considerable decrease in protein synthesis takes place in all tissues. Among the possible causes of the decline of translation in old animals are the modifications of elongation factor-2 (eEF- 2). eEF-2 occupies an essential role in protein synthesis where it catalyzes the ribosomal translocation reaction. eEF-2 is...

A wide range of clinical applications in regenerative medicine were opened decades ago with the discovery of adult stem cells. Highly promising adult stem cells are mesenchymal stem/stromal cells derived from adipose tissue (ADSCs), primarily because of their abundance and accessibility. These cells have multipotent properties and have been...

It has been claimed that oxidative stress and the production of reactive oxygen radicals can contribute to neuron degeneration and might be one factor in the development of different neurological diseases. In our study, we have attempted to clarify how oxidative damage induces dose dependent changes in functional membrane properties of neurons...

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Consejería de Economía, Innovación y Ciencia, Junta de Andalucía P10-CTS-6494

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Senotherapy, a promising therapeutic strategy, has drawn a lot attention recently due to its potential for combating cancer. Senotherapy refers to the targeting of senescent cells to restore tissue homeostasis and mitigate the deleterious effects associated with senescence. Senolytic drugs represent a promising avenue in cancer treatment, with...

Human beings are facing emerging degenerative and cancer diseases, in large part, as a consequence of increased life expectancy. In the near future, researchers will have to put even more effort into fighting these new challenges, one of which will be prevention of cancer while continuing to improve the aging process through this increased life...

La presente invención escribe un método electrofisiológico y un kit consistente en una concentración de 10 µM de hidroperóxido de cumeno puro en líquido cefalorraquídeo para cuantificar los efectos de la toxicidad sobre las propiedades intrínsecas de las neuronas de la corteza motora a los 5 minutos de exposición a la acción tóxica y su uso en...

Background: Parkinson's disease is an irreversible neurodegenerative disease linked to progressive movement disorders and is accompanied by an inflammatory reaction that is believed to contribute to its pathogenesis. Since sensitivity to inflammation is not the same in all brain structures, the aim of this work was to test whether...

Adipose tissue is an important source of adipose derived stem cells (ADSCs). These cells have the potential of being used for certain therapies, in which the main objective is to recover the function of a tissue/organ affected by a disease. In order to contribute to repair of the tissue, these cells should be able to survive and carry out their...

Tras una época de cambios sin precedentes en la educación mundial, tanto escuelas como universidades han adoptado métodos de enseñanza a distancia y virtual, haciendo que las herramientas que los educadores usan para sus cursos evolucionen. Las crisis globales presentes y futuras requieren soluciones científicas avanzadas, por lo que preparar a...

The molecular mechanisms underlying the negative effects of psychological stress on cellular stress during aging and neurodegenerative diseases are poorly understood. The main objective of this study was to test the effect of chronic psychological stress, and the consequent increase of circulating glucocorticoids, on several hippocampal genes...

Cell therapy with adult stem cells is a new battle front for the control of aging. Before being used for this purpose, we need to answer several basic questions about the biochemistry and physiology of these cells. This paper presents some aspects and preliminary results obtained in our laboratory using stem cells from adipose tissue.