NEPITA , I

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Fluorescence lifetime in biological samples is a useful feature to demultiplex the fluorescence signal of spectrally overlapping fluorophores. A problem that frequently occurs when multiple fluorophores are in use is the possible spectral overlap. Therefore, selected fluorophores should be chosen to avoid this issue, consequently constraining...

Optical Nanoscopy deals with designing and developing optical microscopes able to produce images that are rich sources of quantitative information at the nanoscale. This fact offers an unprecedented insight into the molecular mechanisms that govern and determine the fate of living cells, coupled with a large amount of data to be structured. In...

Super-resolution microscopy has been recently applied to understand the 3D topology of chromatin at an intermediated genomic scale (kilobases to a few megabases), as this corresponds to a sub-diffraction spatial scale crucial for the regulation of gene transcription. In this context, polycomb proteins are very renowned gene repressors that...