La regulación redox basada en intercambio disulfuro-ditiol es un mecanismo regulatorio universal que afecta a la conformación y actividad de las proteínas. En organismos heterótrofos, la regulación redox se lleva a cabo mediante un sistema de dos componentes, tioredoxina (Trx) y NADPH tioredoxina reductasa (NTR), el denominado sistema NTR/Trx....
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June 24, 2019 (v1)PublicationUploaded on: March 25, 2023
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June 3, 2019 (v1)Publication
The chloroplast redox network is composed of a complex set of thioredoxins (Trxs), reduced by ferredoxin (Fdx) via a Fdx-dependent Trx reductase (FTR), and an NADPH-dependent Trx reductase with a joint Trx domain, NTRC, which efficiently reduces 2-Cys peroxiredoxins (2-Cys Prxs). Recently, it was proposed that the redox balance of 2-Cys Prxs...
Uploaded on: March 26, 2023 -
April 16, 2019 (v1)Publication
Most redox-regulated chloroplast enzymes are reduced during the day and oxidized during the night. While the reduction mechanism of light-dependent enzymes is well known, the mechanism mediating their oxidation in the dark remains unknown. The thiol-dependent peroxidases, 2-Cys peroxiredoxins (Prxs), play a key role in light-dependent reduction...
Uploaded on: March 27, 2023 -
December 4, 2017 (v1)Publication
Thiol-dependent redox regulation allows the rapid adaptation of chloroplast function to unpredictable changes in light intensity. Traditionally, it has been considered that chloroplast redox regulation relies on photosynthetically reduced ferredoxin (Fd), thioredoxins (Trxs), and an Fd-dependent Trx reductase (FTR), the Fd-FTR-Trxs system,...
Uploaded on: December 4, 2022 -
September 17, 2021 (v1)Publication
In chloroplasts, thiol-dependent redox regulation is linked to light since the disulfide reductase activity of thioredoxins (Trxs) relies on photo-reduced ferredoxin (Fdx). Furthermore, chloroplasts harbor an NADPH-dependent Trx reductase (NTR) with a joint Trx domain, termed NTRC. The activity of these two redox systems is integrated by the...
Uploaded on: December 4, 2022 -
May 14, 2019 (v1)Publication
Light is probably the most important environmental stimulus for plant development. As sessile organisms, plants have developed regulatory mechanisms that allow the rapid adaptation of their metabolism to changes in light availability. Redox regulation based on disulfide-dithiol exchange constitutes a rapid and reversible post-translational...
Uploaded on: March 26, 2023 -
October 27, 2017 (v1)Publication
Thioredoxins (Trxs) play a relevant role in thiol-dependent redox regulation, which allows the rapid adaptation of chloroplast metabolism to unpredictable environmental conditions. In chloroplasts, Trxs use reducing equivalents provided by photoreduced ferredoxin (Fdx) via the action of a ferredoxin-thioredoxin reductase (FTR), thus linking...
Uploaded on: March 26, 2023 -
January 18, 2018 (v1)Publication
In addition to the standard NADPH thioredoxin reductases (NTRs), plants hold a plastidic NTR (NTRC), with a thioredoxin module fused at the C-terminus. NTRC is an efficient reductant of 2-Cys peroxiredoxins (2-Cys Prxs). The interaction of NTRC and chloroplastic thioredoxin x with 2-Cys Prxs has been confirmed in vivo, by bimolecular...
Uploaded on: March 26, 2023 -
December 18, 2017 (v1)Publication
Thiol-dependent redox regulation of enzyme activity plays a central role in the rapid acclimation of chloroplast metabolism to ever-fluctuating light availability. This regulatory mechanism relies on ferredoxin reduced by the photosynthetic electron transport chain, which fuels reducing power to thioredoxins (Trxs) via a ferredoxin-dependent...
Uploaded on: December 4, 2022