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Last name: OLMO FERNÁNDEZ
First name: Alberto
Tesis descargada en Teseo
It is proposed a microscopy for cell culture applications based on impedance sensors. The imagined signals are measured with the Electrical Cell-Substrate Spectroscopy (ECIS) technique, by identifying the cell area. The proposed microscopy allows real-time monitoring inside the incubator, reducing the contamination risk by human manipulation....
Coronary artery disease is one of the most common diseases in developed countries and affects a large part of the population of developing countries. Preventing restenosis in patients with implanted stents is an important current medical problem. The purpose of this work is to analyse the viability of bioimpedance sensing to detect the...
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a disabling and chronic disease, importantly related to the current COVID-19 pandemic. Currently, there are no specific laboratory tests to directly diagnose ME/CFS. In this work, the use of impedance spectroscopy is studied as a potential technique for the diagnosis of ME/CFS. A...
A sinusoidal voltage wave generator is proposed based on the use of micro- processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital...
In this work, a simple protocol based on electrical impedance measures is implemented to detect changes on chemical composition, the microstructure (percentage and size of pores) and the surface modification treatments of metallic implants. The Hewlett-Packard 4395A Agilent impedance analyzer was employed for these measurements. Discs of...
En este trabajo se presenta la integración de contenidos multimedia en la enseñanza de Circuitos Electrónicos Digitales y Estructura de Computadores, del primer curso de Ingeniería Informática en la Universidad de Sevilla. Se han utilizado diferentes herramientas de captura de pantalla y grabación de vídeo en la preparación de material...
In this paper we present the integration of multimedia contents in the teaching of Digital Electronic Circuits and Computer Structure, of the first course of Computer Engineering at the University of Seville. Different tools for screenshot and video recording have been used for the preparation of audiovisual material, integrated in the learning...
Heart-Failure (HF) is among the leading hospitalization causes in modern healthcare systems. In this paper, a method for performing continuous patient monitoring is presented with a focus on low power consumption. A prototype wearable device is being developed at the University of Sevilla to collect measurements. Among the sensing components...
Criopreservación celular por vitrificación con bajas concentraciones de crioprotector.El objeto de la presente invención se refiere a un nuevo procedimiento de criopreservación celular, que consigue la vitrificación de las muestras biológicas con presenci
Porous titanium is a metallic biomaterial with good properties for the clinical repair of cortical bone tissue, although the presence of pores can compromise its mechanical behavior and clinical use. It is therefore necessary to characterize the implant pore size and distribution in a suitable way. In this work, we explore the new use of...
Commercially pure titanium (c.p. Ti) and Ti6Al4V alloys are the most widely used metallic biomaterials in the biomedical sector. However, their high rigidity and the controversial toxicity of their alloying elements often compromise their clinical success. The use of porous β-Titanium alloys is proposed as a solution to these issues. In this...
This paper proposes the application of a cell-microelectrode model in cell biometry experiments, using the cell-electrode area overlap as its main parameter. The model can be applied to cell size identification and cell count, and further extended to study cell growth and dosimetry protocols. Experiments have been conducted in AA8 cell line,...
To follow up the restenosis in arteries stented during an angioplasty is an important current clinical problem. A new approach to monitor the growth of neointimal tissue inside the stent is proposed on the basis of electrical impedance spectroscopy (EIS) sensors and the oscillation-based test (OBT) circuit technique. A mathematical model was...
En este trabajo, se evalúa la impedancia eléctrica de distintas muestras de titanio poroso modificadas superficialmente con un láser de femtosegundo; como método experimental para caracterizar la macroporosidad inherente al proceso de fabricación y el efecto del tratamiento superficial de los discos. El estudio se realiza sobre discos con...
High-throughput data analysis challenges in laboratory automation and lab-on-a-chip devices' applications are continuously increasing. In cell culture monitoring, specifically, the electrical cell-substrate impedance sensing technique (ECIS), has been extensively used for a wide variety of applications. One of the main drawbacks of ECIS is the...
This work proposes a method for the study and real-time monitoring of a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate Impedance Spectroscopy...
This work proposes a method for studying and monitoring in real-time a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate Impedance Spectroscopy...
Heart failure (HF) is a severe disease and one of the most important causes of death in our society nowadays. A significant percentage of patients hospitalized for decompensation of heart failure are readmitted after some weeks or months due to an expected bad and uncontrolled HF evolution due to the lack of the patient supervision in real...
This work describes how is possible the definition of the light hole or lumen in implanted stents affected by restenosis processes using the BioImpedance (BI) as biomarker. The main approach is based on the fact that neointimal tissues implied in restenosis can be detected and measured thanks to their respective conductivity and dielectric...
It is hereby presented a new approach to monitor restenosis in arteries fitted with a stent during an angioplasty. The growth of neointimal tissue is followed up by measuring its bioimpedance with Electrical Impedance Spectroscopy (EIS). Besides, a mathematical model is derived to analytically describe the neointima's histological composition...
Electrical pulse stimulation has an important effect on skeletal muscle development and maturation. However, the methodology for controlling these stimulation parameters to develop in vitro functional skeletal muscle tissues remains to be established. In this work, we have studied the effect of simulated action potentials on the growth and...