OYARZUN , Diego

Cellular metabolism contains intricate arrays of feedback control loops. These are a key mechanism by which cells adapt their metabolism to survive environmental disturbances. Gene regulation, in particular, typically displays complex architectures that combine positive and negative feedback loops between metabolites and enzymatic genes. Yet...

Interactions between gene regulatory networks and metabolism generate a diversity of dynamics, including multistability and oscillatory behavior. Here, we characterize a regulatory mechanism that drives the emergence of periodic oscillations in metabolic networks subject to genetic feedback regulation by pathway intermediates. We employ a...

Cell response heterogeneity upon treatment is a main obstacle in preclinical development of efficacious cancer drugs, due to the emergence of drug-tolerant cells. We have previously developed a single-cell pipeline called Fate-seq to profile drug-tolerant persisters, based on predictions oftheir drug response. To automatize and increase the...

Cell response heterogeneity upon drug treatment is a leading cause of reduced drug efficacy in preclinical cancer research. Although single-cell studies have revealed the depth of cellular heterogeneity observed between in tumor cells, the regulatory impact of cell variability on therapeutic response remains unclear. Here, we present a new ODE...

International audience

Cell response heterogeneity upon treatment is a main obstacle in preclinical development of efficacious cancer drugs, due to the emergence of drug-tolerant cells. We have previously developed a single-cell workflow, Fate-Seq (1,2), to profile drug-tolerant persisters. Fate-Seq is based on the prediction of the drug response of each cells that...

International audience