PENCO , Susanna

To underline the importance of both the ethical issues of alternative approaches to animal testing and the life sciences in the 21st century, LARF-DIMES, at the University of Genova (Italy), is engaged since 2008 in organization of training courses for dissemination of international resources using the over twenty years of experience of its...

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Libro rivolto a studenti di discipline scientifiche, pubblicato da Aracne editrice s.r.l., Roma, 2008

although for most pharmaceutical compounds the final aim is improving human health, almost almost the methods used to identify and purse therapeutic targets and to obtain new potential drugs have traditionally focused on animal models

La complementarità del test in vitro rispetto alle valutazioni in vivo risiede nel fatto di poter arricchire il test in vivo di parametri quantitativi, sensibili, precoci e spesso unici perché non esplorabili direttamente sull"uomo. Il test in vitro può impiegare sistemi biologici semplificati (colture cellulari) o sofisticati (organi e tessuti...

In recent years, the development of research and the increased awareness of our moral duties beyond the human species have pushed the scientific community to revise widely-accepted ontological reductionist views that regard non-human animals as mere things. The new horizons offered by the development of advanced research methods...

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Introduction and objectives. Coinciding with the recent implementation in Italy of the "Directive 2010/63/EU, regarding the protection of animals used for scientific purposes", the Authors would like to analyse the topic of the introduction of ethical committees for animal experimentation in Italy. This paper furthermore aims to underline some...

Purpose A growing evidence in the scientific literature suggests that oxidative damage plays a pathogenic role in primary open-angle glaucoma. Therefore, it is of interest to test whether drugs effective against glaucoma display antioxidant activity. We test the hypothesis that the classic b-blocker therapy for glaucoma with timolol involves...

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Cytotoxic effects related to exposure to benzaldehyde, acetaldehyde, propionaldehyde and valeraldehyde in hepatoma cell lines

In Italy, Law 413/1993 states that public and private Italian Institutions, including academic faculties, are obliged to fully inform workers and students about their right to conscientious objection to scientific or educational activities involving animals, hereafter written as "animal CO". However, little monitoring on the faculties'...

cytotoxic effects of different xenobiotics in three hepatoma cell lines, from rat and human

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We thus applied only one prooxidant stimulus after enriching two hepatoma cell lines (MH1C1 and 7777) with lower concentrations of arachidonic acid (ARA) than those used in previous work, but still able to enhance lipid peroxidation without loss of viability. After pretreatment with ARA and exposure to the prooxidant, the cultured cells were...

Urinary tract infections (UTIs) are frequently polymicrobial diseases mainly sustained by Escherichia coli in association with other opportunistic pathogens. Cystitis and pyelonephritis are usually accompanied by an inflammatory response, which includes neutrophil recruitment. Uropathogenic E. coli possess the ability to evade host defenses,...

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Oxidation antitumor therapy is developed by delivering excess oxidative stress into tumor cells or targetedly disrupting the antioxidant systems. In present study, several markers related to oxidative stress, proliferation and angiogenesis were evaluated in human DLD-1 colon carcinoma, and HECV normal endothelial cells, during exposure to...

On September 29-30, 2016, the 8th edition of the training course on "Alternative Approach to Animal Testing and Cell Cultures, According to European Laws", was held in Genoa, chaired by Prof. Anna Maria Bassi and organized and hosted by the team of Laboratory Analysis and Research in Physiopathology (LARF), Department of Experimental ...

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According to European laws animal testing in cosmetic industry will be prohibited in a few years and it will be replaced by alternative methods based on cell and tissue culture. Many ingredients of cosmetic formulations are potentially causes of skin inflammation and sensibilization. Since cytotoxicity is known, among other factors, to trigger...