POZZOLINI , Marina

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L-Arginine uptake and Ca2+ changes in unstirred platelets activated by thrombin, collagen and Ca2+ ionophore A23187 were evaluated. Thrombin did not affect L-arginine uptake at short incubation times (2-15 min), but at prolonged times slowed down the amino acid transport. Collagen was ineffective, A23187 decreased the L-arginine uptake in a...

There is an increasing demand for alternative sources of collagens, which are needed for a wide variety of applications (food, cosmetics, healthcare). Sponge collagens have unique physico-chemical properties and as a consequence are a promising resource, but sponge collagens are not available in large quantities. A biotechnology-based approach...

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Sponge cells represent a unique model for the study of the molecular evolution of animal metabolism and of the ancestral processes of biomineralization. Among various sponge culture models, the most promising ones are based on the primmorph technique, which allows the production of 3D sponge cell aggregates, easily reared in a laboratory...

The sponge Chondrosia reniformis selectively engulfs siliceous particles that, when in crystalline form, become quickly dissolved in its ectosome. The molecular mechanism, identity, and physiological significance of the cells involved in this process are not completely understood. In the present study, we applied light and electronic...

The possibility to cryopreserve cells allows for wide opportunities of flexible handling of cell cultures from different sponge species. Primmorphs model, a multicellular 3D aggregate formed by dissociated sponge cells, is considered one of the best approaches to establish sponge cell culture but, in spite of the available protocols for...

Prolyl 4-hydroxylase (P4H) catalyzes the hydroxylation of proline residues in collagen. P4H has two functional subunits, α and β. Here, we report the cDNA cloning, characterization, and expression analysis of the α and β subunits of the P4H derived from the marine sponge Chondrosia reniformis. The amino acid sequence of the α subunit is 533...

Background: Inhalation of crystalline silica induces a pulmonary fibrotic degeneration called silicosis caused by the inability of alveolar macrophages to dissolve the crystalline structure of phagocytosed quartz particles. Ascorbic acid is capable of partially dissolving quartz crystals, leading to an increase of soluble silica concentration...

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